Results 41 to 50 of about 614,915 (309)

Next-generation sequencing primers.

, 2023
List of primers used for next-generation sequencing of gDNA extracted from pooled CRISPR activation screen samples. Primers were adapted from Sanson and colleagues [5]. (DOCX)
Lipin Loo (462678), Stuart Turville (7388393), Oltin-Tiberiu Pop (14593003), Matthew A. Waller (14593000), Ann-Kristin Jochum (14593006), Suresh Mathivanan (67730), G. Gregory Neely (6700172), Jason K. K. Low (10182574), Wolfram Jochum (801376), Omar Hasan Ali (14593009), Anupriya Aggarwal (161186), Cesar L. Moreno (3341927), Joel P. Mackay (149378), Daniel Hesselson (437342), Karishma Patel (2332540), Lukas Flatz (251190), Christopher E. Denes (14593012), Alberto Ospina Stella (11084498), Felicity Chung (14593015), Taeyoung Kang (14593018), Rezwan Siddiquee (10882629), Alexander J. Cole (13944095), Zina Hamoudi (7274939)   +22 more
core   +1 more source

Defining Culture Conditions for the Hidden Nitrite-Oxidizing Bacterium Nitrolancea

Frontiers in Microbiology, 2020
Nitrification is a key process for N-removal in engineered and natural environments, but recent findings of novel nitrifying microorganisms with surprising features revealed that our knowledge of this functional guild is still incomplete.
Eva Spieck, Katharina Sass, Sabine Keuter, Sophia Hirschmann, Michael Spohn, Daniela Indenbirken, Linnea F. M. Kop, Sebastian Lücker, Alejandra Giaveno   +8 more
doaj   +1 more source

Neuroinflammation regulates the balance between hippocampal neuron death and neurogenesis in an ex vivo model of thiamine deficiency

Journal of Neuroinflammation, 2022
Background Thiamine (vitamin B1) is a cofactor for enzymes of central energy metabolism and its deficiency (TD) impairs oxidative phosphorylation, increases oxidative stress, and activates inflammatory processes that can lead to neurodegeneration ...
Larissa M. G. Cassiano, Marina S. Oliveira, Jeanne Pioline, Anna C. M. Salim, Roney S. Coimbra   +4 more
doaj   +1 more source

Advancements in Next-Generation Sequencing [PDF]

Annual Review of Genomics and Human Genetics, 2016
The term next-generation sequencing is almost a decade old, but it remains the colloquial way to describe highly parallel or high-output sequencing methods that produce data at or beyond the genome scale. Since the introduction of these technologies, the number of applications and methods that leverage the power of genome-scale sequencing has ...
Shawn E, Levy, Richard M, Myers
openaire   +2 more sources

Targeted genetic testing for familial hypercholesterolaemia using next generation sequencing:a population-based study [PDF]

, 2014
BACKGROUND: Familial hypercholesterolaemia (FH) is a common Mendelian condition which, untreated, results in premature coronary heart disease. An estimated 88% of FH cases are undiagnosed in the UK.
Soutar, Anne K., Shona M Kerr, Jennifer Biggs, Anne K Soutar, Aitman, Timothy J., Porteous, David J, Morris, Andrew D., Morris, Andrew D, Scotland, Generation, Blair H Smith, Generation Scotland, Smith, Blair H, Anna F Dominiczak, Thomas, Ellen, Soutar, Anne K, Vandrovcova, Jana, Kerr, Shona M, Kerr, Shona M., Archie Campbell, Biggs, Jennifer, Norsworthy, Penny J, David J Porteous, Laurence Game, Thomas, Ellen R. A., Game, Laurence, Penny J Norsworthy, Smith, Blair H., Norsworthy, Penny J., Campbell, Archie, Porteous, David J., Aitman, Timothy J, Ellen RA Thomas, Dominiczak, Anna F, Smith, Blair H.; id_orcid, Jana Vandrovcova, ?, ?, Dominiczak, Anna F., Thomas, Ellen RA, Timothy J Aitman, Andrew D Morris   +39 more
core   +1 more source

Next-Generation Sequencing Panel Test in Myeloid Neoplasms and Evaluation with the Clinical Results

, 2022
Objective: Myeloid malignancies are heterogeneous disorders due to defective hematopoiesis and myeloid differentiation of hematopoietic stem/progenitor cell. The molecular landscape of the diseases is complex.
Gulden Sincan, Cigdem Yuce Kahraman, Sincan, Gulden, Tatar, Abdulgani, Abdulgani Tatar, Yuce Kahraman, Cigdem   +5 more
core   +1 more source

The Evolution of Post-Vaccine G8P[4] Group a Rotavirus Strains in Rwanda; Notable Variance at the Neutralization Epitope Sites

Pathogens, 2023
Africa has a high level of genetic diversity of rotavirus strains, which is suggested to be a possible reason contributing to the suboptimal effectiveness of rotavirus vaccines in this region.
Peter N. Mwangi, Robyn-Lee Potgieter, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Jason M. Mwenda, Milton T. Mogotsi, Kebareng Rakau, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +13 more
doaj   +1 more source

Next-Generation Sequencing Strategies [PDF]

Cold Spring Harbor Perspectives in Medicine, 2018
More than a decade ago, the term "next-generation" sequencing was coined to describe what was, at the time, revolutionary new methods to sequence RNA and DNA at a faster pace and cheaper cost than could be performed by standard bench-top protocols. Since then, the field of DNA sequencing has evolved at a rapid pace, with new breakthroughs allowing ...
Shawn E, Levy, Braden E, Boone
openaire   +2 more sources

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Schroth Gary P, Krüger Detlev H., Kentaro Yoshida, Kahl, Guenter, Kahl, G., Fujibe, Takahiro, Krueger, Detlev H, Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Barrero, Roberto A, Yoshida Kentaro, Luo, S., Barrero Roberto A, Terauchi Ryohei, Albertyn Zayed, Luo, Shujun, Shujun Luo, Kimura, Eiji, Barrero, R.A., Schroth, Gary P., Terauchi, Ryohei, Detlev H Krüger, Yoshida Kentaro, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Yoshida, Kentaro, Kahl Günter, Matsumura, Hideo, Luo Shujun, Krueger Detlev H, Albertyn Zayed, Gary P. Schroth, Kahl Guenter, Matsumura, Hideo, Fujibe Takahiro, Matsumura Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Luo Shujun, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Albertyn, Zayed, Matsumura Hideo, Barrero Roberto A., Schroth, Gary P, Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Kimura Eiji, Roberto A. Barrero, Günter Kahl   +68 more
core   +1 more source

Next‐generation sequencing for mitochondrial disorders [PDF]

British Journal of Pharmacology, 2014
A great deal of our understanding of mitochondrial function has come from studies of inherited mitochondrial diseases, but still majority of the patients lack molecular diagnosis. Furthermore, effective treatments for mitochondrial disorders do not exist.
Carroll CJ, Brilhante V, Suomalainen A.
openaire   +2 more sources