Results 31 to 40 of about 5,736,241 (346)

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg, Biskup, S., Vogl, Ina, Hirv, K., Greif, P.A., Kotschote, S., Gehring, A., Kuhn, M., Stuhrmann, Manfred, Bergmann, C., Eck, Sebastian H., Biskup, Saskia, Metzeler, Klaus H., Greif, Philipp A., Stuhrmann, M., Bolz, Hanno Joern, Benet-Pages, Anna, Hirv, Kaimo, Benet-Pagès, A., Klein, H.G., Vogl, I., Bolz, H.J., Kuhn, Marius, Kotschote, Stefan, Gehring, Andrea, Bergmann, Carsten, Metzeler, K.H., Eck, S.H.   +27 more
core   +1 more source

Genomic Analysis of G2P[4] Group A Rotaviruses in Zambia Reveals Positive Selection in Amino Acid Site 7 of Viral Protein 3

Viruses, 2023
The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi, Robyn-Lee Potgieter, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Milton T. Mogotsi, Nonkululeko Magagula, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +10 more
doaj   +1 more source

RADSeq: next-generation population genetics [PDF]

, 2010
Next-generation sequencing technologies are making a substantial impact on many areas of biology, including the analysis of genetic diversity in populations.
Davey, John L, M. L. Blaxter, Blaxter, Mark W, J. W. Davey   +3 more
core   +1 more source

Comprehensive Detection of Candidate Pathogens in the Lower Respiratory Tract of Pediatric Patients With Unexpected Cardiopulmonary Deterioration Using Next-Generation Sequencing [PDF]

, 2020
Objectives: Next-generation sequencing has been applied to the investigation of microorganisms in several clinical settings. We investigated the infectious etiologies in respiratory specimens from pediatric patients with unexpected cardiopulmonary ...
Suzuki, Takako, Kazuhiro Horiba, Makoto Yamaguchi, Jun-ichi Kawada, Horiba, Kazuhiro, Toshihiko Okumura, Takanari Ikeyama, Okumura, Toshihiko, Ikeyama, Takanari, Shinji Kawabe, Kawabe, Shinji, Ito, Yoshinori, Yuka Torii, Takeuchi, Suguru, Kawada, Jun-ichi, Sho Wada, Yamaguchi, Makoto, Torii, Yuka, Yoshinori Ito, Suguru Takeuchi, Wada, Sho, Takako Suzuki   +21 more
core   +1 more source

Uncovering the First Atypical DS-1-like G1P[8] Rotavirus Strains That Circulated during Pre-Rotavirus Vaccine Introduction Era in South Africa

Pathogens, 2020
Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi, Milton T. Mogotsi, Sebotsana P. Rasebotsa, Mapaseka L. Seheri, M. Jeffrey Mphahlele, Valantine N. Ndze, Francis E. Dennis, Khuzwayo C. Jere, Martin M. Nyaga   +8 more
doaj   +1 more source

A framework for variation discovery and genotyping using next-generation DNA sequencing data

Nature Genetics, 2011
Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution.
M. DePristo, E. Banks, Ryan Poplin, K. Garimella, Jared Maguire, Christopher Hartl, A. Philippakis, G. Del Angel, M. Rivas, Matthew G. Hanna, A. McKenna, T. Fennell, Andrew M. Kernytsky, A. Sivachenko, K. Cibulskis, S. Gabriel, David A. Altshuler, M. Daly   +17 more
semanticscholar   +1 more source

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Schroth Gary P, Krüger Detlev H., Kentaro Yoshida, Kahl, Guenter, Kahl, G., Fujibe, Takahiro, Krueger, Detlev H, Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Barrero, Roberto A, Yoshida Kentaro, Luo, S., Barrero Roberto A, Terauchi Ryohei, Albertyn Zayed, Luo, Shujun, Shujun Luo, Kimura, Eiji, Barrero, R.A., Schroth, Gary P., Terauchi, Ryohei, Detlev H Krüger, Yoshida Kentaro, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Yoshida, Kentaro, Kahl Günter, Matsumura, Hideo, Luo Shujun, Krueger Detlev H, Albertyn Zayed, Gary P. Schroth, Kahl Guenter, Matsumura, Hideo, Fujibe Takahiro, Matsumura Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Luo Shujun, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Albertyn, Zayed, Matsumura Hideo, Barrero Roberto A., Schroth, Gary P, Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Kimura Eiji, Roberto A. Barrero, Günter Kahl   +68 more
core   +1 more source

A genome-wide survey of mutations in the Jurkat cell line

BMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su   +4 more
doaj   +1 more source

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

Nucleic Acids Research, 2012
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth, E. Pruesse, Timmy Schweer, J. Peplies, C. Quast, M. Horn, F. Glöckner   +6 more
semanticscholar   +1 more source

Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells

Frontiers in Immunology, 2022
The transcriptomic profile of a cell population can now be studied at the cellular level using single-cell mRNA sequencing (scRNA-seq). This novel technique provides the unprecedented opportunity to explore the cellular composition of the bronchoalveolar
Sophie E. Sage, Pamela Nicholson, Laureen M. Peters, Tosso Leeb, Tosso Leeb, Vidhya Jagannathan, Vinzenz Gerber   +6 more
doaj   +1 more source