Results 31 to 40 of about 1,096,418 (305)

Whole-Genome Analyses Identifies Multiple Reassortant Rotavirus Strains in Rwanda Post-Vaccine Introduction

Viruses, 2021
Children in low-and middle-income countries, including Rwanda, experience a greater burden of rotavirus disease relative to developed countries. Evolutionary mechanisms leading to multiple reassortant rotavirus strains have been documented over time ...
Sebotsana Rasebotsa, Jeannine Uwimana, Milton T. Mogotsi, Kebareng Rakau, Nonkululeko B. Magagula, Mapaseka L. Seheri, Jason M. Mwenda, M. Jeffrey Mphahlele, Saheed Sabiu, Richard Mihigo, Leon Mutesa, Martin M. Nyaga   +11 more
doaj   +1 more source

NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

PLoS ONE, 2012
Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence data. However, quality control (QC) of sequence data generated from these technologies is extremely important for meaningful downstream ...
Ravi K. Patel, Mukesh Jain
semanticscholar   +1 more source

Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts

Genome Biology, 2009
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer ...
J. Levin, Michael F Berger, Xian Adiconis, P. Rogov, A. Melnikov, T. Fennell, C. Nusbaum, Levi A. Garraway, A. Gnirke   +8 more
semanticscholar   +1 more source

Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center

Microbiome, 2019
Background Viruses play an important role in ecosystems, including the built environment (BE). While numerous studies have characterized bacterial and fungal microbiomes in the BE, few have focused on the viral microbiome (virome).
Aaron J. Prussin, Pedro J. Torres, John Shimashita, Steven R. Head, Kyle J. Bibby, Scott T. Kelley, Linsey C. Marr   +6 more
doaj   +1 more source

Next-generation sequencing: insights to advance clinical investigations of the microbiome

Journal of Clinical Investigation, 2022
Next-generation sequencing (NGS) technology has advanced our understanding of the human microbiome by allowing for the discovery and characterization of unculturable microbes with prediction of their function.
Caroline R. Wensel, J. Pluznick, S. Salzberg, C. Sears   +3 more
semanticscholar   +1 more source

Bioinformatics and Next- generation Sequencing [PDF]

Klinicka onkologie, 2015
Next-generation sequencing technologies are currently well-established in the research field and progressively find their way towards clinical applications. Sequencers produce vast amounts of data and therefore bioinformatics methods are needed for processing.
A, Krejčí, P, Müller, B, Vojtěšek
openaire   +2 more sources

Next-generation sequencing in dermatology

Frontiers in Medicine, 2023
Over the past decade, Next-Generation Sequencing (NGS) has advanced our understanding, diagnosis, and management of several areas within dermatology. NGS has emerged as a powerful tool for diagnosing genetic diseases of the skin, improving upon traditional PCR-based techniques limited by significant genetic heterogeneity associated with these disorders.
Andrew D. King, Hany Deirawan, Paytra A. Klein, Bahar Dasgeb, Catherine I. Dumur, Darius R. Mehregan   +5 more
openaire   +3 more sources

Genomic Analysis of Rwandan G9P[8] Rotavirus Strains Pre- and Post-RotaTeq^® Vaccine Reveals Significant Distinct Sub-Clustering in a Post-Vaccination Cohort

Viruses, 2023
Although the introduction of rotavirus vaccines has substantially contributed to the reduction in rotavirus morbidity and mortality, concerns persist about the re-emergence of variant strains that might alter vaccine effectiveness in the long term.
Robyn-Lee Potgieter, Peter N. Mwangi, Milton T. Mogotsi, Jeannine Uwimana, Leon Mutesa, Narcisse Muganga, Didier Murenzi, Lisine Tusiyenge, Mapaseka L. Seheri, A. Duncan Steele, Jason M. Mwenda, Martin M. Nyaga   +11 more
doaj   +1 more source

Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads—a baiting and iterative mapping approach

Nucleic Acids Research, 2013
We present an in silico approach for the reconstruction of complete mitochondrial genomes of non-model organisms directly from next-generation sequencing (NGS) data—mitochondrial baiting and iterative mapping (MITObim). The method is straightforward even
C. Hahn, L. Bachmann, Bastien Chevreux
semanticscholar   +1 more source

Recommendations for the use of next-generation sequencing (NGS) for patients with metastatic cancers: A report from the ESMO Precision Medicine Working Group.

Annals of Oncology, 2020
Next-generation sequencing (NGS) allows sequencing a high number of nucleotides in a short time frame at an affordable cost. While this technology has been widely implemented, there are no recommendations from scientific societies about their use in ...
F. Mosele, J. Remon, J. Mateo, C. B. Westphalen, F. Barlesi, M. Lolkema, N. Normanno, A. Scarpa, Mark E. Robson, F. Meric-Bernstam, N. Wagle, A. Stenzinger, J. Bonastre, A. Bayle, S. Michiels, I. Bièche, E. Rouleau, S. Jezdic, J. Douillard, J. Reis-Filho, R. Dienstmann, F. André   +21 more
semanticscholar   +1 more source