Dumpster diving for diatom plastid 16S rRNA genes
PeerJ, 2021 High throughput sequencing is improving the efficiency of monitoring diatoms, which inhabit and support aquatic ecosystems across the globe. In this study, we explored the potential of a standard V4 515F-806RB primer pair in recovering diatom plastid 16S rRNA sequences.
Bonfantine, Krista L. +4 more
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Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]
, 2013 The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan +23 more
core +1 more source
Comparison of 16S rRNA Gene Quantification with Selected Inflammatory Markers and Culture Method in Septic Arthritis [PDF]
, 2023 This study aims to determine the types of causative organism, the utility of synovial procalcitonin (PCT), C-Reactive Protein (CRP) and bacterial 16S rRNA gene-based RT-PCR and their comparison with conventional culture results in patients with ...
Ahmad Fadzli Bin Solong +5 more
core +1 more source
Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]
, 2011 The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère +13 more
core +2 more sources
Amylase and 16S rRNA genes from a hyperthermophilic archaebacterium [PDF]
Journal of Applied Microbiology, 1999 A hyperthermophilic and amylolytic prokaryote, designated Rt3, was isolated from a thermal spring near Rotorua, New Zealand. The 16S rRNA gene of Rt3 was cloned and sequenced with the aim of determining its phylogenetic affiliations. The phylogenetic analysis of this sequence, which included a selection of archaebacterial and eubacterial 16S rRNA ...
Jones, R. A. +4 more
openaire +3 more sources
Yersinia spp. Identification Using Copy Diversity in the Chromosomal 16S rRNA Gene Sequence. [PDF]
PLoS ONE, 2016 API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g.
Huijing Hao +9 more
doaj +1 more source
Microbiology Spectrum, 2022 Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin +7 more
doaj +1 more source
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]
, 2012 Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA +30 more
core +4 more sources
Microbiome, 2020 Background The low cost of 16S rRNA gene sequencing facilitates population-scale molecular epidemiological studies. Existing computational algorithms can resolve 16S rRNA gene sequences into high-resolution amplicon sequence variants (ASVs), which ...
Isabel F. Escapa +6 more
doaj +1 more source
Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]
, 2001 A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen +8 more
core +3 more sources