Results 21 to 30 of about 183,216 (261)

OTU analysis using metagenomic shotgun sequencing data. [PDF]

open access: yesPLoS ONE, 2012
Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
doaj   +1 more source

16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences analysis of the genus Myxococcus [PDF]

open access: yesFEMS Microbiology Letters, 2008
Phylogenetic relationships of the species belonging to the genus Myxococcus were elucidated based on the sequences of 16S rRNA genes and 16S-23S rRNA gene internal transcribed spacer (ITS) regions. The Myxococcus species were consequently classified into four distinct groups.
Mika Miyashita   +3 more
openaire   +2 more sources

Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

open access: yesData in Brief, 2021
The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes   +6 more
doaj   +1 more source

Reconstructing 16S rRNA genes in metagenomic data [PDF]

open access: yesBioinformatics, 2015
Abstract Metagenomic data, which contains sequenced DNA reads of uncultured microbial species from environmental samples, provide a unique opportunity to thoroughly analyze microbial species that have never been identified before. Reconstructing 16S ribosomal RNA, a phylogenetic marker gene, is usually required to analyze the composition
Cheng Yuan 0001   +3 more
openaire   +2 more sources

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

open access: yesF1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source

Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons ofVibrio parahaemolyticus [PDF]

open access: yesFEMS Microbiology Letters, 2005
The genome sequence of a strain of Vibrio parahaemolyticus holds 11 copies of rRNA operons (rrn) with identical 16S rRNA genes (rrs). Conversely, the species type strain contains two rrs classes differing in 10 nucleotide sites within a short segment of 25 bp.
González Escalona, Narjol   +2 more
openaire   +4 more sources

Comparison of 16S rRNA gene sequences of genus Methanobrevibacter [PDF]

open access: yesBMC Microbiology, 2004
The phylogeny of the genus Methanobrevibacter was established almost 25 years ago on the basis of the similarities of the 16S rRNA oligonucleotide catalogs. Since then, many 16S rRNA gene sequences of newly isolated strains or clones representing the genus Methanobrevibacter have been deposited.
Patole Milind S   +6 more
openaire   +3 more sources

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters

open access: yesmSphere, 2021
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj   +1 more source

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

open access: yesMicrobiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

Amylase and 16S rRNA genes from a hyperthermophilic archaebacterium [PDF]

open access: yesJournal of Applied Microbiology, 1999
A hyperthermophilic and amylolytic prokaryote, designated Rt3, was isolated from a thermal spring near Rotorua, New Zealand. The 16S rRNA gene of Rt3 was cloned and sequenced with the aim of determining its phylogenetic affiliations. The phylogenetic analysis of this sequence, which included a selection of archaebacterial and eubacterial 16S rRNA ...
Jones, R. A.   +4 more
openaire   +3 more sources

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