Results 1 to 10 of about 152,159 (258)
Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing [PDF]
mSphere, 2021 In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings.
Isabel Abellan-Schneyder +7 more
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Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments
mSystems, 2019 Microbial communities are commonly studied using culture-independent methods, such as 16S rRNA gene sequencing. However, one challenge in accurately characterizing microbial communities is exogenous bacterial DNA contamination, particularly in low ...
Lisa Karstens +7 more
doaj +5 more sources
Application of 16S rRNA gene sequencing in Helicobacter pylori detection [PDF]
PeerJ, 2020 Helicobacter pylori is one of the major stomach microbiome components, promoting development of inflammation and gastric cancer in humans. H. pylori has a unique ability to transform into a coccoidal form which is difficult to detect by many diagnostic ...
Aleksander Szymczak +6 more
doaj +4 more sources
Phylogenetic inference of Coxiella burnetii by 16S rRNA gene sequencing. [PDF]
PLoS ONE, 2017 Coxiella burnetii is a human pathogen that causes the serious zoonotic disease Q fever. It is ubiquitous in the environment and due to its wide host range, long-range dispersal potential and classification as a bioterrorism agent, this microorganism is ...
Heather P McLaughlin +10 more
doaj +4 more sources
metaSPARSim: a 16S rRNA gene sequencing count data simulator [PDF]
BMC Bioinformatics, 2019 Background In the last few years, 16S rRNA gene sequencing (16S rDNA-seq) has seen a surprisingly rapid increase in election rate as a methodology to perform microbial community studies.
Ilaria Patuzzi +4 more
doaj +3 more sources
Progress in the 16S rRNA Gene Sequencing in Forensic Science [PDF]
Fayixue Zazhi, 2018 Forensic microorganism is one of the hotspots of forensic science research. Due to its conservatism and specificity, the 16S rRNA gene is found to be an ideal marker for forensic identification.
SONG Guo-qing, CAO Yu, LI Hui，et al.
doaj +2 more sources
Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens
Microbiology Spectrum, 2022 16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat +2 more
doaj +1 more source
Data in Brief, 2021 The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes +6 more
doaj +1 more source
16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis [PDF]
PeerJ, 2022 Background The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing.
Ke Zhang +4 more
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Comparison of 16S rRNA gene sequences of genus Methanobrevibacter [PDF]
BMC Microbiology, 2004 The phylogeny of the genus Methanobrevibacter was established almost 25 years ago on the basis of the similarities of the 16S rRNA oligonucleotide catalogs. Since then, many 16S rRNA gene sequences of newly isolated strains or clones representing the genus Methanobrevibacter have been deposited.
Patole Milind S +6 more
openaire +3 more sources