Results 41 to 50 of about 250,877 (332)

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

Microbiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source

Differential Functional Constraints Cause Strain-Level Endemism in Polynucleobacter Populations. [PDF]

, 2016
The adaptation of bacterial lineages to local environmental conditions creates the potential for broader genotypic diversity within a species, which can enable a species to dominate across ecological gradients because of niche flexibility.
Geeta Rijal, Herbert Ssegane, Iratxe Zarraonaindia, Jack A. Gilbert, Janet K. Jansson, Jarrad T. Hampton-Marcell, M. Cristina Negri, Naseer Sangwan, Tifani W. Eshoo   +8 more
core   +6 more sources

Establishment and assessment of an amplicon sequencing method targeting the 16S-ITS-23S rRNA operon for analysis of the equine gut microbiome

Scientific Reports, 2021
Microbial communities are commonly studied by using amplicon sequencing of part of the 16S rRNA gene. Sequencing of the full-length 16S rRNA gene can provide higher taxonomic resolution and accuracy.
Yuta Kinoshita, Hidekazu Niwa, Eri Uchida-Fujii, Toshio Nukada   +3 more
doaj   +1 more source

Concatenated 16S rRNA sequence analysis improves bacterial taxonomy [version 3; peer review: 2 approved]

F1000Research, 2023
Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj   +1 more source

Reconciliation between operational taxonomic units and species boundaries [PDF]

, 2017
The development of high-throughput sequencing technologies has revolutionised the field of microbial ecology via 16S rRNA gene amplicon sequencing approaches.
Boon, Nico, Kerckhof, Frederiek-Maarten, Leys, Natalie, Monsieurs, Pieter, Mysara, Mohamed, Props, Ruben, Raes, Jeroen, Vandamme, Peter   +7 more
core   +2 more sources

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]

, 2016
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J., Clooney, Adam G, Cotter, Paul D., Fouhy, Fiona, STANTON, CATHERINE   +4 more
core   +2 more sources

The efficacy of partial 16S rRNA gene sequencing for precise determination of phylogenetic relatedness among Salmonellae

Scientific African, 2021
More than 2,600 Salmonella enterica serovars have been described based on the expression of the somatic and flagellar antigens. For epidemiological tracing, the use of Salmonellae serotyping is inefficient because strains within the same serovar could be
I.M.T. Fadlalla, M.E. Hamid, A.G. ARahim, M.A. Osman   +3 more
doaj   +1 more source

IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis. [PDF]

, 2020
Dupilumab is a fully human antibody to interleukin-4 receptor α that improves the signs and symptoms of moderate to severe atopic dermatitis (AD). To determine the effects of dupilumab on Staphylococcus aureus colonization and microbial diversity on the ...
Ardeleanu, Marius, Bissonnette, Robert, Callewaert, Chris, Gallo, Richard L, Graham, Neil MH, Guttman-Yassky, Emma, Hamilton, Jennifer D, Hultsch, Thomas, Knight, Rob, Kosciolek, Tomasz, Kotol, Paul, Krueger, James, Menter, Alan, Nakatsuji, Teruaki, Pirozzi, Gianluca, Silverberg, Jonathan I, Vrbanac, Alison   +16 more
core   +2 more sources

Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]

, 2011
Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Bogert, B., van den, Vos, W.M., de, Zoetendal, E.G., Kleerebezem, M.   +3 more
core   +1 more source

Catheter-Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing [PDF]

Journal of Clinical Microbiology, 2002
ABSTRACT We describe the application of 16S rRNA gene sequencing in defining two cases of catheter-related Microbacterium bacteremia. In the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia.
Yuen, KY, Lau, SKP, Woo, PCY, Woo, GKS
openaire   +4 more sources