Results 41 to 50 of about 3,149,654 (297)

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen, Jong, S., de, Kowalchuk, G.A., Stephen, J.R., De Jong, S., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +8 more
core   +3 more sources

The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates [PDF]

, 2006
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA
Lee, J.L., Tae Jung Kim, Jae Il Lee, Kim, T.J.   +3 more
core   +1 more source

Information about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification.

PLoS ONE, 2019
Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, its use remains limited due to the presence of variable copy numbers with sequence variation ...
Jerald Conrad Ibal, Huy Quang Pham, Chang Eon Park, Jae-Ho Shin   +3 more
doaj   +1 more source

Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing

World Journal of Gastroenterology, 2020
This study determined the composition and diversity of intestinal microflora in patients with colorectal adenoma (CRA), which may provide precedence for investigating the role of intestinal microflora in the pathogenesis of colorectal tumors, the composition of intestinal microflora closely related to CRA, and further validating the possibility of ...
Wang, Wen-Jia, Zhou, You-Lian, He, Jie, Feng, Zhi-Qiang, Zhang, Long, Lai, Xiao-Bo, Zhou, Jun-Xiao, Wang, Hong   +7 more
openaire   +2 more sources

Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine [PDF]

, 2009
BACKGROUND: Variations in the composition of the human intestinal microbiota are linked to diverse health conditions. High-throughput molecular technologies have recently elucidated microbial community structure at much higher resolution than was ...
Vos, W.M., de, Willem M de Vos, O\u27Toole, Paul W., Smidt, H., O'Sullivan, O., Janne Nikkilä, Claesson Marcus J., de Vos Willem M., Hauke Smidt, O'Sullivan, Orla, Marchesi, Julian Roberto, O\u27Sullivan, Orla, Ross R. Paul, Smidt, Hauka, Ross, R. Paul, Ross, R. P., Paul W O'Toole, Wang, Qiong, Orla O'Sullivan, O'Sullivan Orla, de Vos, W.M., Qiong Wang, Nikkilä Janne, O'Toole, Paul W., Claesson, M.J., Marchesi, J.R., O'Toole, P.W., Claesson, Marcus J., O'Toole, P. W., Wang, Q., de Vos, Willem M., Claesson, M. J., Julian R Marchesi, O'Toole Paul W., Marchesi, Julian R., Smidt, Hauke, Ross, R.P., Smidt Hauke, Nikkila, Janne, R Paul Ross, Marchesi Julian R., Ross, R Paul, Wang Qiong, De Vos, W. M., Nikkilä, J., Marcus J Claesson, O'Sullivan, Orla, O'Toole, Paul W.   +47 more
core   +1 more source

Phylogenetic Position of Riemerella anatipestifer Based on 16S rRNA Gene Sequences [PDF]

International Journal of Systematic Bacteriology, 1997
Riemerella anatipestifer, the causative agent of septicemia anserum exsudativa (also called new duckling disease), belongs to the family Flavobacteriaceae of gram-negative bacteria. We determined the DNA sequences of the rrs genes encoding the 16S rRNAs of four R. anatipestifer strains by directly sequencing PCR-amplified rrs genes.
Subramaniam, S., Chua, K.-L., Tan, H.-M., Loh, H., Kuhnert, P., Frey, J.   +5 more
openaire   +3 more sources

De novo species identification using 16S rRNA gene nanopore sequencing [PDF]

PeerJ, 2020
Nanopore sequencing is rapidly becoming more popular for use in various microbiota-based applications. Major limitations of current approaches are that they do not enable de novo species identification and that they cannot be used to verify species ...
Inga Leena Angell, Morten Nilsen, Karin C. Lødrup Carlsen, Kai-Håkon Carlsen, Gunilla Hedlin, Christine M. Jonassen, Benjamin Marsland, Björn Nordlund, Eva Maria Rehbinder, Carina Saunders, Håvard Ove Skjerven, Anne Cathrine Staff, Cilla Söderhäll, Riyas Vettukattil, Knut Rudi   +14 more
doaj   +2 more sources

Molecular detection of bacteria from a possible material oral origin in neonatal gastric aspirates obtained from complicated pregnancies [PDF]

, 2011
PhDIt has been suggested that periodontal disease, a disease that affects the supporting tissues of the teeth, represents a risk factor for adverse pregnancy outcomes.
Gonzales Marin, Cecilia
core   +4 more sources

[Identification of mycobacteria by sequencing of rpoB gene and 16S rRNA].

Kekkaku : [Tuberculosis], 2006
To classify a specific Mycobacterium among various mycobacteria utilizing sequencing of rpoB gene. To classify mycobacteria not identified by DNA-DNA hybridization (DDH) using sequencing of rpoB and 16S rRNA gene.Classification of 106 Mycobacteria strains, one Nocardia strain, one Rhodococcus strain, four Gordona strains was made by using partial ...
KAZUMI, Yuko, MAEDA, Shinji, SUGAWARA, Isamu   +2 more
openaire   +2 more sources

Comparison of two approaches for the classification of 16S rRNA gene sequences

Journal of Medical Microbiology, 2014
The use of 16S rRNA gene sequences for microbial identification in clinical microbiology is accepted widely, and requires databases and algorithms. We compared a new research database containing curated 16S rRNA gene sequences in combination with the lca (lowest common ancestor) algorithm (RDB-LCA) to a commercially available 16S rDNA Centroid approach.
Sonia, Chatellier, Nathalie, Mugnier, Françoise, Allard, Bertrand, Bonnaud, Valérie, Collin, Alex, van Belkum, Jean-Baptiste, Veyrieras, Stefan, Emler   +7 more
openaire   +2 more sources