Results 41 to 50 of about 3,369,418 (253)

Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?

Poultry Science
Bacteria are the major component of poultry gastrointestinal tract (GIT) microbiota and play an important role in host health, nutrition, physiology regulation, intestinal development, and growth.
Joshua M. Lyte, Mitiku M. Seyoum, Diana Ayala, Jannigje G. Kers, Valentina Caputi, Timothy Johnson, Li Zhang, Joshua Rehberger, Guolong Zhang, Sami Dridi, Brett Hale, Jean E. De Oliveira, Daniel Grum, Alexandra H. Smith, Michael Kogut, Steven C. Ricke, Anne Ballou, Bill Potter, Monika Proszkowiec-Weglarz   +18 more
doaj   +1 more source

Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules. [PDF]

PLoS ONE, 2016
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes.
James E McDonald, Niels Larsen, Andrea Pennington, John Connolly, Corrin Wallis, David J Rooks, Neil Hall, Alan J McCarthy, Heather E Allison   +8 more
doaj   +1 more source

Comparing the Efficacy of MALDI-TOF MS and Sequencing-Based Identification Techniques (Sanger and NGS) to Monitor the Microbial Community of Irrigation Water

Microorganisms, 2023
In order to intensify and guarantee the agricultural productivity and thereby to be able to feed the world’s rapidly growing population, irrigation has become very important. In parallel, the limited water resources lead to an increase in usage of poorly
Botond Bendegúz Surányi, Benjamin Zwirzitz, Csilla Mohácsi-Farkas, Tekla Engelhardt, Konrad J. Domig   +4 more
doaj   +1 more source

Evaluating oxalate-degrading Lactobacillus spp. for their ability to be used as probiotics in the treatment of kidney stone disease [PDF]

, 2010
Includes abstract.Includes bibliographical references (leaves 73-95).Although the direct cause of kidney stone formation is not known, reports have suggested it is probably a multifactorial disease.
Kabanda, Siti M
core   +1 more source

The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates [PDF]

, 2006
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA
Lee, J.L., Tae Jung Kim, Jae Il Lee, Kim, T.J.   +3 more
core   +1 more source

16S rRNA sequencing

, 2023
   16S rRNA ...
Tong Zou (14354496)
core   +1 more source

Phylogenetic inference of Coxiella burnetii by 16S rRNA gene sequencing. [PDF]

PLoS ONE, 2017
Coxiella burnetii is a human pathogen that causes the serious zoonotic disease Q fever. It is ubiquitous in the environment and due to its wide host range, long-range dispersal potential and classification as a bioterrorism agent, this microorganism is ...
Heather P McLaughlin, Blake Cherney, Janetta R Hakovirta, Rachael A Priestley, Andrew Conley, Andrew Carter, David Hodge, Segaran P Pillai, Linda M Weigel, Gilbert J Kersh, David Sue   +10 more
doaj   +1 more source

Linking 16S rRNA Gene Classification to amoA Gene Taxonomy Reveals Environmental Distribution of Ammonia-Oxidizing Archaeal Clades in Peatland Soils

mSystems, 2021
A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang, Alexandre Bagnoud, Rafael I. Ponce-Toledo, Melina Kerou, Micha Weil, Christa Schleper, Tim Urich   +6 more
doaj   +1 more source

Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Jong, S., de, Kowalchuk, G.A., Stephen, J.R., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +6 more
core   +1 more source

SituSeq: an offline protocol for rapid and remote Nanopore 16S rRNA amplicon sequence analysis

ISME Communications, 2023
Microbiome analysis through 16S rRNA gene sequencing is a crucial tool for understanding the microbial ecology of any habitat or ecosystem. However, workflows require large equipment, stable internet, and extensive computing power such that most of the ...
Jackie Zorz, Carmen Li, Anirban Chakraborty, Daniel A. Gittins, Taylor Surcon, Natasha Morrison, Robbie Bennett, Adam MacDonald, Casey R. J. Hubert   +8 more
doaj   +1 more source