Results 21 to 30 of about 3,149,654 (297)

16S classifier: a tool for fast and accurate taxonomic classification of 16S rRNA hypervariable regions in metagenomic datasets. [PDF]

PLoS ONE, 2015
The diversity of microbial species in a metagenomic study is commonly assessed using 16S rRNA gene sequencing. With the rapid developments in genome sequencing technologies, the focus has shifted towards the sequencing of hypervariable regions of 16S ...
Nikhil Chaudhary, Ashok K Sharma, Piyush Agarwal, Ankit Gupta, Vineet K Sharma   +4 more
doaj   +1 more source

16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences analysis of the genus Myxococcus [PDF]

FEMS Microbiology Letters, 2008
Phylogenetic relationships of the species belonging to the genus Myxococcus were elucidated based on the sequences of 16S rRNA genes and 16S-23S rRNA gene internal transcribed spacer (ITS) regions. The Myxococcus species were consequently classified into four distinct groups.
Mika Miyashita, Takeshi Sakane, Ken-ichiro Suzuki, Yasuyoshi Nakagawa   +3 more
openaire   +2 more sources

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters

mSphere, 2021
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj   +1 more source

Identification of Comamonas species using 16S rRNA gene sequence [PDF]

Bioinformation, 2009
A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas.
Vimlesh, Yadav, Satya, Prakash, Shipra, Srivastava, Praveen Chandra, Verma, Vijayta, Gupta, Vaishali, Basu, Anil Kumar, Rawat   +6 more
openaire   +2 more sources

Quantifying dominant bacterial genera detected in metagenomic data from fish eggs and larvae using genus‐specific primers

MicrobiologyOpen, 2022
The goal of this study was to design genus‐specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon‐based sequencing of the 16S rRNA gene in fish‐related samples and surrounding water. Efficient genus‐specific
Babak Najafpour, Patricia I. S. Pinto, A. V. M. Canario, Deborah M. Power   +3 more
doaj   +1 more source

A Phylogenetic Analysis of the Genus Nocardia with 16S rRNA Gene Sequences [PDF]

International Journal of Systematic Bacteriology, 1995
Partial sequences of the 16S rRNA genes of the type strains of nine species of the genus Nocardia were determined following the isolation and cloning of the amplified genes. These sequences were aligned with the sequences of representatives of the genera Corynebacterium, Gordona, Mycobacterium, Rhodococcus, and Tsukamurella, and phylogenetic trees were
J, Chun, M, Goodfellow
openaire   +2 more sources

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J., Penn, Charles W, Cogan Tristan, Pallen, Mark J., Charles W Penn, Chrystala Constantinidou (161352), Charles W. Penn (161371), Sergeant, MJ, Cogan, Tristan, Tristan Cogan (161360), Constantinidou Chrystala, Constantinido, C, Mark J Pallen, Tristan Cogan, Martin J. Sergeant (161344), Constantinidou, Chrystala, Sergeant, Martin J, Penn Charles W., Pallen, Mark J, Sergeant, Martin J., Chrystala Constantinidou, Mark J. Pallen (8904), Penn, CW, Pallen, MJ, Penn, Charles W., Pallen Mark J., Martin J Sergeant, Cogan, TA   +27 more
core   +2 more sources

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]

, 2012
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Kowalchuk, GA, Quince, Christopher, Christopher Quince, de Hollander, M, Quince Christopher, Vos, M., Pijl, Agata S., Vos Michiel, Michiel Vos, Vos, M, Pijl, A.S., Agata S. Pijl (183429), Quince, C., Pijl Agata S., Kowalchuk, G.A., Mattias de Hollander, Hollander, M. de, Michiel Vos (63922), Mattias de Hollander (183437), Vos, Michiel, Quince, C, George A Kowalchuk, Kowalchuk George A., Pijl, AS, de Hollander, Mattias, George A. Kowalchuk (183442), Agata S Pijl, Kowalchuk, George A., Christopher Quince (178494), de Hollander, M., de Hollander Mattias   +30 more
core   +4 more sources

Engineering CRISPR/Cas9 to mitigate abundant host contamination for 16S rRNA gene-based amplicon sequencing

Microbiome, 2020
Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj   +1 more source