, 2023 Sequencing the 16S rRNA gene from feces of mice from the following groups: non-diabetic (VH-PBS); unsupplemented diabetic (STZ-PBS); diabetic supplemented with Akkermansia muciniphila (STZ-Akk)
Fernandes Rodrigues, Vanessa +1 more
core +2 more sources
BMC Microbiology, 2021 Background Species-level genetic characterization of complex bacterial communities has important clinical applications in both diagnosis and treatment. Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene has proven to be a powerful strategy for the ...
Yoshiyuki Matsuo +13 more
doaj +1 more source
, 2017 of A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq ...
Eric de Muinck (4230820) +4 more
core +8 more sources
A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]
, 2012 Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Quince, Christopher +24 more
core +2 more sources
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J. +27 more
core +2 more sources
Results 16s rRNA gene amplification and sequencing.
, 2022 This table contains the 16s rRNA gene amplification and sequencing results presented in Table 5 of the manuscript. (XLSX)
Nicole Wente (13014753) +4 more
core +1 more source
PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]
, 2012 As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205) +7 more
core +2 more sources
Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]
, 2011 The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère +13 more
core +2 more sources
Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]
, 2011 Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Vos, W.M., de +7 more
core +1 more source
Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing [PDF]
, 2012 Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W. +27 more
core +1 more source