Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]
PLoS ONE, 2013 The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Swee Hoe Ong +7 more
doaj +6 more sources
Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing. [PDF]
World J Gastrointest SurgLu ZX +8 more
europepmc +2 more sources
16S rRNA gene sequencing for bacterial identification and infectious disease diagnosis
Biochemical and Biophysical Research CommunicationsAbstract16S rRNA gene sequence is the most common housekeeping genetic marker to study bacterial phylogeny and taxonomy. Therefore, 16S rRNA gene sequencing has the potential to identify novel bacteria and diagnose bacteria. This study compared 16S rRNA gene sequencing with conventional PCR for bacterial identification and disease diagnosis.
Zhuang Zhu, George Fei Zhang
exaly +3 more sources
Diagnostic Yield and Impact on Antimicrobial Management of 16S rRNA Testing of Clinical Specimens
Microbiology Spectrum, 2022 16S rRNA gene sequencing is increasingly used in clinical practice for bacterial identification of clinical specimens. However, studies on its applicability to direct clinical specimens are limited. Here, we studied the diagnostic yield and impact of 16S
Pruke Eamsakulrat +2 more
doaj +1 more source
16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis [PDF]
PeerJ, 2022 Background The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing.
Ke Zhang +4 more
doaj +2 more sources
The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies [PDF]
, 2013 Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced.
De Vos, Paul +4 more
core +11 more sources
Data in Brief, 2021 The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples ...
Timothy E. Mattes +6 more
doaj +1 more source
Frontiers in Microbiology, 2023 The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier +9 more
doaj +1 more source
Virtual metagenome reconstruction from 16S rRNA gene sequences [PDF]
Nature Communications, 2012 Microbial ecologists have investigated roles of species richness and diversity in a wide variety of ecosystems. Recently, metagenomics have been developed to measure functions in ecosystems, but this approach is cost-intensive. Here we describe a novel method for the rapid and efficient reconstruction of a virtual metagenome in environmental microbial ...
Shujiro, Okuda +4 more
openaire +2 more sources
Benchmarking of 16S rRNA gene databases using known strain sequences [PDF]
Bioinformation, 2021 16S rRNA gene analysis is the most convenient and robust method for microbiome studies. Inaccurate taxonomic assignment of bacterial strains could have deleterious effects as all downstream analyses rely heavily on the accurate assessment of microbial taxonomy.
Dixit, Kunal +7 more
openaire +2 more sources