Results 31 to 40 of about 3,149,654 (297)
Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling
Frontiers in Cellular and Infection Microbiology, 2022 Accessibility to next-generation sequencing (NGS) technologies has enabled the profiling of microbial communities living in distinct habitats. 16S ribosomal RNA (rRNA) gene sequencing is widely used for microbiota profiling with NGS technologies.
Vitor Heidrich +9 more
doaj +1 more source
Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]
, 2011 The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère +13 more
core +2 more sources
PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]
, 2012 As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205) +7 more
core +2 more sources
Scientific Reports, 2021 Microbial communities are commonly studied by using amplicon sequencing of part of the 16S rRNA gene. Sequencing of the full-length 16S rRNA gene can provide higher taxonomic resolution and accuracy.
Yuta Kinoshita +3 more
doaj +1 more source
F1000Research, 2023 Background: Microscopic, biochemical, molecular, and computer-based approaches are extensively used to identify and classify bacterial populations.
Bobby Paul
doaj +1 more source
Beating Naive Bayes at Taxonomic Classification of 16S rRNA Gene Sequences [PDF]
Frontiers in Microbiology, 2021 Naive Bayes classifiers (NBC) have dominated the field of taxonomic classification of amplicon sequences for over a decade. Apart from having runtime requirements that allow them to be trained and used on modest laptops, they have persistently provided class-topping classification accuracy.
Ziemski, Michal; id_orcid0000-0001-6285-8852 +3 more
openaire +4 more sources
Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]
, 2011 Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Vos, W.M., de +9 more
core +1 more source
, 2023 Sequencing the 16S rRNA gene from feces of mice from the following groups: non-diabetic (VH-PBS); unsupplemented diabetic (STZ-PBS); diabetic supplemented with Akkermansia muciniphila (STZ-Akk)
Fernandes Rodrigues, V
core +2 more sources
Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing [PDF]
, 2012 Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W. +20 more
core +1 more source
Scientific African, 2021 More than 2,600 Salmonella enterica serovars have been described based on the expression of the somatic and flagellar antigens. For epidemiological tracing, the use of Salmonellae serotyping is inefficient because strains within the same serovar could be
I.M.T. Fadlalla +3 more
doaj +1 more source