Results 41 to 50 of about 183,216 (261)

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

International Journal of Mycobacteriology, 2012
Background: Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent.
Yuko Kazumi, Satoshi Mitarai
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

Phylogenies of the 16S rRNA gene and its hypervariable regions lack concordance with core genome phylogenies

Microbiome, 2022
Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in species delineation, and now widely in microbiome studies. However, the gene suffers from intragenomic heterogeneity, and reports of recombination and an unreliable ...
Hayley B. Hassler, Brett Probert, Carson Moore, Elizabeth Lawson, Richard W. Jackson, Brook T. Russell, Vincent P. Richards   +6 more
doaj   +1 more source

Prevalence of 16S rRNA methylase genes among β-lactamase-producing Enterobacteriaceae clinical isolates in Saudi Arabia [PDF]

Libyan Journal of Medicine, 2014
Background: Co production of 16S rRNA methylases gene and β-Lactamase gene among Enterobacteriaceae isolates conferring resistance to both therapeutic options has serious implications for clinicians worldwide.
Yazeed A. Al Sheikh, Mohammed Ali M. Marie, James John, Lakshmana Gowda Krishnappa, Khaled Homoud M. Dabwab   +4 more
doaj   +1 more source

Taxonomic and Characterization Methods of Streptomyces: A Review

Progress in Microbes and Molecular Biology, 2018
The 16S ribosomal RNA gene is the gold standard for taxonomic identification and phylogenetic study of most of the bacteria. However, the resolution of 16S rRNA gene is found to be insufficient to distinguish closely related Streptomyces species within ...
Jodi Woan-Fei Law, Kei-Xian Tan, Sunny Hei Wong, Nurul-Syakima Ab Mutalib, Learn-Han Lee   +4 more
doaj   +1 more source

The human gut microbiome across the life course

FEBS Letters, EarlyView.
Despite significant individual variation and continuous change throughout life, the human gut microbiome follows some life stage‐specific trends. This article provides a brief overview of how gut microbiome composition shifts across different phases of life. Created in BioRender. Özkurt, E. (2026) https://BioRender.com/8q4nrnc.
Alise J. Ponsero, Basak Bahcivanci, Antonietta Hayhoe, Animesh Acharjee, Ezgi Özkurt   +4 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Simultaneous measurement of 16S-rRNA and pre-16S-rRNA as a strategy to monitor clinical tuberculosis

Frontiers in Antibiotics
BackgroundCulture-based biomarkers of TB treatment response monitoring, e.g., Mycobacterial Growth Indicatory Tube (MGIT), are compromised when bacteria enter a non-replicating persister phase limiting the measurement of antibiotic efficacy and ...
Evelin Dombay, Wilber Sabiiti, Daniela Alferes de Lima Headley, M. Bonifác Légrády, Nina van Campen, Sanne Zweijpfennig, Martin J. Boeree, Derek J. Sloan, Stephen H. Gillespie   +8 more
doaj   +1 more source

Latitudinal variation in the potential activity of Atlantic Ocean bacterioplankton revealed through 16S rRNA and 16S rRNA gene metabarcoding

Frontiers in Marine Science, 2023
The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen, Kimberley E. Bird, Kimberley E. Bird, J. Colin Murrell, Michael Cunliffe, Michael Cunliffe   +5 more
doaj   +1 more source