Results 41 to 50 of about 327,715 (297)
Specificity Between Lactobacilli And Hymenopteran Hosts Is The Exception Rather Than The Rule [PDF]
, 2013 Lactobacilli (Lactobacillales: Lactobacillaceae) are well known for their roles in food fermentation, as probiotics, and in human health, but they can also be dominant members of the microbiota of some species of Hymenoptera (ants, bees, and wasps ...
Cannone, Jamie J. +5 more
core +1 more source
Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]
, 2020 Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J. +8 more
core +2 more sources
16S rRNA gene Library Preparation Protocol v1
, 2023 16S rRNA gene library preparation protocol including the following steps: MPure extraction of Bacterial DNA, Manual 16S indexed primer PCR, library clean up with beads, quibit DNA quantification, equimolar mix construction, agarose gel DNA concentration and gel clean up to produce a library ready for Illumina sequencing.
dinesh.aggarwal not provided +3 more
openaire +1 more source
PCR-generated artefact from 16S rRNA gene-specific primers [PDF]
FEMS Microbiology Letters, 2005 Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex ...
Catherine A, Osborne +3 more
openaire +2 more sources
MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze and improve the accuracy of taxonomic classification of skin bacteria with MinION ...
Miquel Rozas +4 more
openaire +4 more sources
Defining reference sequences for Nocardia species by similarity and clustering analyses of 16S rRNA gene sequence data. [PDF]
PLoS ONE, 2011 BACKGROUND: The intra- and inter-species genetic diversity of bacteria and the absence of 'reference', or the most representative, sequences of individual species present a significant challenge for sequence-based identification.
Manal Helal +5 more
doaj +1 more source
Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]
, 2018 Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A +3 more
core +1 more source
BMC Microbiology, 2021 Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host ...
M. Singer +9 more
doaj +1 more source
The Journal of General and Applied Microbiology, 2015 In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Naoshi, Fujimoto +10 more
openaire +3 more sources
PCR primers to amplify 16S rRNA genes from cyanobacteria [PDF]
Applied and Environmental Microbiology, 1997 We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms
Nübel, U. +2 more
openaire +3 more sources