Results 51 to 60 of about 2,798,395 (304)

Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing [PDF]

, 2012
Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W., Shawn W. Polson (139348), Craig W. Herbold (139346), Charles K Lee, Ian R McDonald, S Craig Cary, K Eric Wommack, K. Eric Wommack (139352), Charles K. Lee (139341), Ian R. McDonald (139355), Herbold, Craig W., Lee, Charles Kai-Wu, Shawn W Polson, Shannon J. Williamson (38225), Williamson, Shannon J., Cary, S. Craig, S. Craig Cary (133811), Craig W Herbold, Shannon J Williamson, McDonald, Ian R., Wommack, K. Eric   +20 more
core   +1 more source

Defining reference sequences for Nocardia species by similarity and clustering analyses of 16S rRNA gene sequence data. [PDF]

PLoS ONE, 2011
BACKGROUND: The intra- and inter-species genetic diversity of bacteria and the absence of 'reference', or the most representative, sequences of individual species present a significant challenge for sequence-based identification.
Manal Helal, Fanrong Kong, Sharon C A Chen, Michael Bain, Richard Christen, Vitali Sintchenko   +5 more
doaj   +1 more source

The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis

BMC Microbiology, 2021
Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host ...
M. Singer, R. Koedooder, M. P. Bos, L. Poort, S. Schoenmakers, P. H. M. Savelkoul, J. S. E. Laven, J. D. de Jonge, S. A. Morré, A. E. Budding   +9 more
doaj   +1 more source

Louse (Insecta : Phthiraptera) mitochondrial 12S rRNA secondary structure is highly variable [PDF]

, 2002
Lice are ectoparasitic insects hosted by birds and mammals. Mitochondrial 12S rRNA sequences obtained from lice show considerable length variation and are very difficult to align.
Page, R.D.M., Cruickshank, R., Johnson, K.P.   +2 more
core   +1 more source

Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

Emerging Infectious Diseases, 2002
In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi, Anne M. Whitney, Leonard W. Mayer, Roger Morey, Arnold Steigerwalt, Ariana Boras, Robin S. Weyant, Tanja Popovic   +7 more
doaj   +1 more source

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

International Journal of Mycobacteriology, 2012
Background: Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent.
Yuko Kazumi, Satoshi Mitarai
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

Инфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov, S. N. Zhdanova, E. A. Orlova, P. A. Khromova, N. L. Belkova, V. V. Sinkov, I. G. Kondratov   +6 more
doaj   +1 more source

Phylogenies of the 16S rRNA gene and its hypervariable regions lack concordance with core genome phylogenies

Microbiome, 2022
Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in species delineation, and now widely in microbiome studies. However, the gene suffers from intragenomic heterogeneity, and reports of recombination and an unreliable ...
Hayley B. Hassler, Brett Probert, Carson Moore, Elizabeth Lawson, Richard W. Jackson, Brook T. Russell, Vincent P. Richards   +6 more
doaj   +1 more source

Paenibacillus brasilensis sp nov., a novel nitrogen-fixing species isolated from the maize rhizosphere in Brazil [PDF]

, 2002
Sixteen nitrogen-fixing strains isolated from the rhizosphere of maize planted in Cerrado soil, Brazil, which showed morphological and biochemical characteristics similar to the gas-forming Paenibacillus spp., were phenotypically and genetically ...
Seldin, L., Weid, I., von der, Elsas, J.D., van, Duarte, G.F.   +3 more
core   +1 more source

Prevalence of 16S rRNA methylase genes among β-lactamase-producing Enterobacteriaceae clinical isolates in Saudi Arabia [PDF]

Libyan Journal of Medicine, 2014
Background: Co production of 16S rRNA methylases gene and β-Lactamase gene among Enterobacteriaceae isolates conferring resistance to both therapeutic options has serious implications for clinicians worldwide.
Yazeed A. Al Sheikh, Mohammed Ali M. Marie, James John, Lakshmana Gowda Krishnappa, Khaled Homoud M. Dabwab   +4 more
doaj   +1 more source