Results 71 to 80 of about 327,715 (297)

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Latitudinal variation in the potential activity of Atlantic Ocean bacterioplankton revealed through 16S rRNA and 16S rRNA gene metabarcoding

Frontiers in Marine Science, 2023
The activities of bacterioplankton sustain open ocean biogeochemical and ecological processes, however, little is known about the activity of specific bacterioplankton, especially related to their biogeography across oceanic scales.
Ro Allen, Kimberley E. Bird, Kimberley E. Bird, J. Colin Murrell, Michael Cunliffe, Michael Cunliffe   +5 more
doaj   +1 more source

Differential Functional Constraints Cause Strain-Level Endemism in Polynucleobacter Populations. [PDF]

, 2016
The adaptation of bacterial lineages to local environmental conditions creates the potential for broader genotypic diversity within a species, which can enable a species to dominate across ecological gradients because of niche flexibility.
Geeta Rijal, Herbert Ssegane, Iratxe Zarraonaindia, Jack A. Gilbert, Janet K. Jansson, Jarrad T. Hampton-Marcell, M. Cristina Negri, Naseer Sangwan, Tifani W. Eshoo   +8 more
core   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniae and comparison with four other species specific PCR assays [PDF]

, 2010
Background: Streptococcus pneumoniae is one of the most frequently encountered pathogens in humans but its differentiation from closely related but less pathogenic streptococci remains a challenge.
Nabil Abdullah El Aila, Stefan Emler, Tarja Kaijalainen, Thierry De Baere, Bart Saerens, Elife Alkan, Pieter Deschaght, Rita Verhelst, Mario Vaneechoutte, A Ortqvist, L Spanjaard, D Rozkiewicz, JC Arbique, CA Dias, S Nunes, SI Aguiar, T Kaijalainen, N Suzuki, TO Messmer, R Verhelst, T Kaijalainen, G Carvalho Mda, GM Abdeldaim, M Vaneechoutte, S Rintamaki, KE Morrison, D Llull, JC McAvin, N Suzuki, ER Keith, RW Bentley   +30 more
core   +3 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

Microbiology Spectrum
Precise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj   +1 more source

DNA barcoding of Cynoglossus arel using mitochondrial COI and 16S rRNA genes

Indian Journal of Animal Sciences, 2020
DNA barcoding is not a substitute for taxonomy; however, it does provide a powerful tool to aid species identifications and focus on future taxonomic research efforts.
CHITRA SOMAN, H N ANJANEYAPPA, B T NAVEEN KUMAR, T N DEVANAND, R P SATHISH, MALATHI SHEKAR   +5 more
doaj   +1 more source

Assessment of the microbial communities associated with white syndrome and brown jelly syndrome in aquarium corals [PDF]

, 2013
Bacterial and ciliate assemblages associated with aquarium corals displaying white syndrome (WS) and brown jelly syndrome (BJS) were investigated. Healthy (n = 10) and diseased corals (WS n = 18; BJS n = 3) were analysed for 16S rRNA gene bacterial ...
Bythell, John C., Craggs, Jamie, Robson, James, Sweet, Michael J.   +3 more
core   +1 more source

Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors [PDF]

, 2008
The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel ...
Altinbas, M., Freeman, S.A., Hollingsworth, J., Sierra-Alvarez, R., Smidt, H., Stams, A.J.M.   +5 more
core   +2 more sources