Results 31 to 40 of about 2,986,865 (305)

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters

mSphere, 2021
16S rRNA gene sequencing has engendered significant interest in studying microbial communities. There has been tension between trying to classify 16S rRNA gene sequences to increasingly lower taxonomic levels and the reality that those levels were ...
Patrick D. Schloss
doaj   +1 more source

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay, Hibberd Martin Lloyd, Kukkillaya V.U., Nagarajan Niranjan, Ong Swee Hoe, Low L., Ho E.X.P., Lay Christophe, Ho Eliza Xin Pei, Wilm A., Low Louie, Nagarajan N., Hibberd M.L., Lay C., Ong S.H., Kukkillaya Vinutha Uppoor, Wilm Andreas   +39 more
core   +1 more source

Plastid 16S rRNA gene diversity among eukaryotic picophytoplankton sorted by flow cytometry from the South Pacific Ocean [PDF]

, 2011
The genetic diversity of photosynthetic picoeukaryotes was investigated in the South East Pacific Ocean. Genetic libraries of the plastid 16S rRNA gene were constructed on picoeukaryote populations sorted by flow cytometry, using two different primer ...
Cécile Lepère, Vaulot, Daniel, Lepère Cécile, Scanlan, David J, David J. Scanlan, Xiao Li Shi, Shi, Xiao Li, Scanlan, David J., Lepère, Cécile, Scanlan David J., Shi Xiao Li, Vaulot Daniel, David J Scanlan, Daniel Vaulot   +13 more
core   +2 more sources

Molecular cloning of the rRNA genes of Prevotella bryantii B14

Acta Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Amylase and 16S rRNA genes from a hyperthermophilic archaebacterium [PDF]

Journal of Applied Microbiology, 1999
A hyperthermophilic and amylolytic prokaryote, designated Rt3, was isolated from a thermal spring near Rotorua, New Zealand. The 16S rRNA gene of Rt3 was cloned and sequenced with the aim of determining its phylogenetic affiliations. The phylogenetic analysis of this sequence, which included a selection of archaebacterial and eubacterial 16S rRNA ...
Jones, R. A., Jermiin, L. S., Easteal, S., Patel, B. K. C., Beacham, I. R.   +4 more
openaire   +3 more sources

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity [PDF]

, 2012
Background: The 16S rRNA gene is the gold standard in molecular surveys of bacterial and archaeal diversity, but it has the disadvantages that it is often multiple-copy, has little resolution below the species level and cannot be readily interpreted in ...
Quince, Christopher, Christopher Quince, Quince Christopher, Vos, M., Pijl, Agata S., Vos Michiel, Michiel Vos, Pijl, A.S., Agata S. Pijl (183429), Quince, C., Pijl Agata S., Kowalchuk, G.A., Mattias de Hollander, Michiel Vos (63922), Mattias de Hollander (183437), Vos, Michiel, George A Kowalchuk, Kowalchuk George A., de Hollander, Mattias, George A. Kowalchuk (183442), Agata S Pijl, Kowalchuk, George A., Christopher Quince (178494), de Hollander, M., de Hollander Mattias   +24 more
core   +2 more sources

Construction of habitat-specific training sets to achieve species-level assignment in 16S rRNA gene datasets

Microbiome, 2020
Background The low cost of 16S rRNA gene sequencing facilitates population-scale molecular epidemiological studies. Existing computational algorithms can resolve 16S rRNA gene sequences into high-resolution amplicon sequence variants (ASVs), which ...
Isabel F. Escapa, Yanmei Huang, Tsute Chen, Maoxuan Lin, Alexis Kokaras, Floyd E. Dewhirst, Katherine P. Lemon   +6 more
doaj   +1 more source

Yersinia spp. Identification Using Copy Diversity in the Chromosomal 16S rRNA Gene Sequence. [PDF]

PLoS ONE, 2016
API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g.
Huijing Hao, Junrong Liang, Ran Duan, Yuhuang Chen, Chang Liu, Yuchun Xiao, Xu Li, Mingming Su, Huaiqi Jing, Xin Wang   +9 more
doaj   +1 more source

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

Microbiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin, Chung-Hsu Lai, Shang-Yi Lin, Ching-Chi Lee, Nan-Yao Lee, Po-Yu Liu, Chih-Hui Yang, Yi-Han Huang   +7 more
doaj   +1 more source

Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Jong, S., de, Kowalchuk, G.A., Stephen, J.R., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +6 more
core   +1 more source