Results 31 to 40 of about 2,798,395 (304)

Microbial signatures in amniotic fluid at preterm birth and association with bronchopulmonary dysplasia

Respiratory Research, 2023
Background Microbiome dysbiosis can have long-lasting effects on our health and induce the development of various diseases. Bronchopulmonary dysplasia (BPD) is a multifactorial disease with pre- and postnatal origins including intra-amniotic infection as
Birte Staude, Silvia Gschwendtner, Tina Frodermann, Frank Oehmke, Thomas Kohl, Susanne Kublik, Michael Schloter, Harald Ehrhardt   +7 more
doaj   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Sergeant Martin J., Penn, Charles W, Cogan Tristan, Pallen, Mark J., Charles W Penn, Chrystala Constantinidou (161352), Charles W. Penn (161371), Sergeant, MJ, Cogan, Tristan, Tristan Cogan (161360), Constantinidou Chrystala, Constantinido, C, Mark J Pallen, Tristan Cogan, Martin J. Sergeant (161344), Constantinidou, Chrystala, Sergeant, Martin J, Penn Charles W., Pallen, Mark J, Sergeant, Martin J., Chrystala Constantinidou, Mark J. Pallen (8904), Penn, CW, Pallen, MJ, Penn, Charles W., Pallen Mark J., Martin J Sergeant, Cogan, TA   +27 more
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Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes [PDF]

Applied and Environmental Microbiology, 2006
From near to far , from here to there , funny things are everywhere. —Dr. Seuss, One Fish Two Fish Red Fish Blue Fish In 1909, H. Joel Conn ([25][1]) expressed the hope that methods would soon be at hand by which the significance of the different bacteria present in any soil could ...
openaire   +2 more sources

Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]

, 2011
Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Vos, W.M., de, Bartholomeus van den Bogert, Kleerebezem, M., van den Bogert, B., Michiel Kleerebezem, Willem M. de Vos, Zoetendal, E.G., Bogert, B., van den, de Vos, W.M., Erwin G. Zoetendal   +9 more
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Performance of Conventional Urine Culture Compared to 16S rRNA Gene Amplicon Sequencing in Children with Suspected Urinary Tract Infection

Microbiology Spectrum, 2021
Because some organisms causing urinary tract infection (UTI) may be difficult to culture, examination of bacterial gene sequences in the urine may provide a more accurate view of bacteria present during a UTI.
Christopher W. Marshall, Marcia Kurs-Lasky, Christi L. McElheny, Sophia Bridwell, Hui Liu, Nader Shaikh   +5 more
doaj   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Fujimoto, Naoshi, Mizuno, Keigo, Yokoyama, Tomoki, Ohnishi, Akihiro, Suzuki, Masaharu, Watanabe, Satoru, Komatsu, Kenji, Sakata, Yoichi, Kishida, Naohiro, Akiba, Michihiro, Matsukura, Satoko   +10 more
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Copy number of the 16S rRNA gene in Rickettsia prowazekii [PDF]

Journal of Bacteriology, 1993
The obligate intracellular parasite, Rickettsia prowazekii, is a slowly growing bacterium with a doubling time of 8 to 12 h. The copy number of the 16S rRNA gene in the rickettsial chromosome was determined to be one. Genomic DNA from R. prowazekii was digested either by a variety of restriction enzymes known not to cut at any site in the rickettsial ...
H, Pang, H H, Winkler
openaire   +2 more sources

Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA [PDF]

, 2001
The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Anneke Keijzer, Van Elsas, J.D., Duineveld, B.M., Kowalchuk, G.A., Van Veen, J.A., George A. Kowalchuk, Jan Dirk van Elsas, Keijzer, A., Veen, J.A., van, Bernadette M. Duineveld, Elsas, J.D., van, Johannes A. van Veen   +11 more
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Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii [PDF]

Journal of Bacteriology, 1996
The control of rRNA synthesis in the etiological agent of epidemic typhus, Rickettsia prowazekii, a slowly growing obligate intracytoplasmic bacterium, was investigated. Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only a single copy, was controlled by a single promoter region, and the site for the initiation of transcription
H, Pang, H H, Winkler
openaire   +2 more sources

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +2 more sources