Results 31 to 40 of about 183,216 (261)

Molecular cloning of the rRNA genes of Prevotella bryantii B14

open access: yesActa Agriculturae Slovenica, 2000
Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained.
Matjaž PETERKA, Gorazd AVGUŠTIN
doaj   +1 more source

Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii [PDF]

open access: yesJournal of Bacteriology, 1996
The control of rRNA synthesis in the etiological agent of epidemic typhus, Rickettsia prowazekii, a slowly growing obligate intracytoplasmic bacterium, was investigated. Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only a single copy, was controlled by a single promoter region, and the site for the initiation of transcription
H, Pang, H H, Winkler
openaire   +2 more sources

Dumpster diving for diatom plastid 16S rRNA genes

open access: yesPeerJ, 2021
High throughput sequencing is improving the efficiency of monitoring diatoms, which inhabit and support aquatic ecosystems across the globe. In this study, we explored the potential of a standard V4 515F-806RB primer pair in recovering diatom plastid 16S rRNA sequences.
Bonfantine, Krista L.   +4 more
openaire   +3 more sources

Construction of habitat-specific training sets to achieve species-level assignment in 16S rRNA gene datasets

open access: yesMicrobiome, 2020
Background The low cost of 16S rRNA gene sequencing facilitates population-scale molecular epidemiological studies. Existing computational algorithms can resolve 16S rRNA gene sequences into high-resolution amplicon sequence variants (ASVs), which ...
Isabel F. Escapa   +6 more
doaj   +1 more source

Yersinia spp. Identification Using Copy Diversity in the Chromosomal 16S rRNA Gene Sequence. [PDF]

open access: yesPLoS ONE, 2016
API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g.
Huijing Hao   +9 more
doaj   +1 more source

Effect of Intragenomic Sequence Heterogeneity among Multiple 16S rRNA Genes on Species Identification of Elizabethkingia

open access: yesMicrobiology Spectrum, 2022
Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin   +7 more
doaj   +1 more source

Microbial signatures in amniotic fluid at preterm birth and association with bronchopulmonary dysplasia

open access: yesRespiratory Research, 2023
Background Microbiome dysbiosis can have long-lasting effects on our health and induce the development of various diseases. Bronchopulmonary dysplasia (BPD) is a multifactorial disease with pre- and postnatal origins including intra-amniotic infection as
Birte Staude   +7 more
doaj   +1 more source

Performance of Conventional Urine Culture Compared to 16S rRNA Gene Amplicon Sequencing in Children with Suspected Urinary Tract Infection

open access: yesMicrobiology Spectrum, 2021
Because some organisms causing urinary tract infection (UTI) may be difficult to culture, examination of bacterial gene sequences in the urine may provide a more accurate view of bacteria present during a UTI.
Christopher W. Marshall   +5 more
doaj   +1 more source

Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes [PDF]

open access: yesApplied and Environmental Microbiology, 2006
From near to far , from here to there , funny things are everywhere. —Dr. Seuss, One Fish Two Fish Red Fish Blue Fish In 1909, H. Joel Conn ([25][1]) expressed the hope that methods would soon be at hand by which the significance of the different bacteria present in any soil could ...
openaire   +2 more sources

Copy number of the 16S rRNA gene in Rickettsia prowazekii [PDF]

open access: yesJournal of Bacteriology, 1993
The obligate intracellular parasite, Rickettsia prowazekii, is a slowly growing bacterium with a doubling time of 8 to 12 h. The copy number of the 16S rRNA gene in the rickettsial chromosome was determined to be one. Genomic DNA from R. prowazekii was digested either by a variety of restriction enzymes known not to cut at any site in the rickettsial ...
H, Pang, H H, Winkler
openaire   +2 more sources

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