Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
Applied and Environmental Microbiology, 2006 A 16S rRNA gene database (http://greengenes.lbl.gov ) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies.
T. DeSantis +9 more
semanticscholar +1 more source
Identification of Host-Specific Bacteroidales 16S rDNA Sequences from Human Sewage and Ruminant Feces [PDF]
, 2011 The need to identify the source of fecal contamination of water has led to the development of various fecal source identification methods, a field known as microbial source tracking (MST). One promising method of MST focuses on fecal members of the order
Allsop +25 more
core +1 more source
A Quantitative Sequencing Framework for Absolute Abundance Measurements of Mucosal and Lumenal Microbial Communities [PDF]
, 2020 A fundamental goal in microbiome studies is determining which microbes affect host physiology. Standard methods for determining changes in microbial taxa measure relative, rather than absolute abundances.
Barlow, Jacob T. +2 more
core +1 more source
Characterization of the human tumor microbiome reveals tumor-type specific intra-cellular bacteria
OncoImmunology, 2020 Many characteristics of cancer such as proliferation, survival, progression, immunogenicity, sensitivity, and resistance to therapy are not just endogenously driven by the tumor cells themselves, but are greatly affected by their interaction with the ...
Ilana Livyatan +3 more
doaj +1 more source
Microbiome, 2020 Background High-throughput sequencing of bacterial 16S rRNA gene (16S-seq) is a useful and common method for studying bacterial community structures. However, contamination of the 16S rRNA genes from the mitochondrion and plastid hinders the sensitive ...
Luyang Song, Kabin Xie
doaj +1 more source
PHYLOGENY OF SOME MIDDLE AMERICAN PITVIPERS BASED ON A CLADISTIC ANALYSIS OF MITOCHONDRIAL 12S AND 16S DNA SEQUENCE INFORMATION [PDF]
, 1999 The cladistic relationships of several Middle American pitvipers representing the genera Bothrops (sensu stricto), Bothriechis, Cerrophidion, Lachesis and Porthidium were determined using mitochondrial 12S and 16S DNA sequence information.
Crother, Brian I. +2 more
core +2 more sources
Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.
Genome Research, 2011 Bacterial diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification ...
B. Haas +14 more
semanticscholar +1 more source
Environmental Microbiome, 2020 Sequencing of 16S rRNA genes has become a powerful technique to study microbial communities and their responses towards changing environmental conditions in various ecosystems.
F. Wemheuer +6 more
semanticscholar +1 more source
Animal Biodiversity and Conservation, 2019 La diversidad oculta en las identificaciones basadas en la morfología de especies invasoras de amplia distribución: el caso de la “bien conocida” hidromedusa Craspedacusta sowerbii Lankester 1880 El número relativamente escaso de características ...
J. A. Oualid +5 more
doaj +1 more source
High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan +4 more
core +3 more sources