Strategy for the identification of micro-organisms producing food and feed products : bacteria producing food enzymes as study case [PDF]
, 2020 Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products.
De Keersmaecker, Sigrid C. J. +8 more
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Mitochondrial DNA. Part B. Resources, 2018 Microhyla ornata, a species originally described from the southwest coast of India in 1841, was long reported to be wide-ranging throughout South, Southeast, and East Asia. Although the name M.
Sonali Garg +3 more
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Animal Biodiversity and Conservation, 2019 La diversidad oculta en las identificaciones basadas en la morfología de especies invasoras de amplia distribución: el caso de la “bien conocida” hidromedusa Craspedacusta sowerbii Lankester 1880 El número relativamente escaso de características ...
J. A. Oualid +5 more
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OTU analysis using metagenomic shotgun sequencing data. [PDF]
PLoS ONE, 2012 Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides
Xiaolin Hao, Ting Chen
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Variable Region Sequences Influence 16S rRNA Performance
Microbiology Spectrum, 2023 16S rRNA gene sequences are commonly analyzed for taxonomic and phylogenetic studies because they contain variable regions that can help distinguish different genera. However, intra-genus distinction using variable region homology is often impossible due
Nikhil Bose, Sean D. Moore
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Microbial Composition and Diversity of High-demand Street-vended Foods in Ecuador
Journal of Food ProtectionDeveloping countries such as Ecuador carry a heavy food safety burden but reports on the microbiological quality of their foods are scarce. In this investigation, the microbial diversity of 10 high-risk and mass-consumption street-vended foods including ...
Byron Díaz Cárdenas +6 more
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High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]
, 2012 The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan +4 more
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Identification of Host-Specific Bacteroidales 16S rDNA Sequences from Human Sewage and Ruminant Feces [PDF]
, 2011 The need to identify the source of fecal contamination of water has led to the development of various fecal source identification methods, a field known as microbial source tracking (MST). One promising method of MST focuses on fecal members of the order
Allsop +25 more
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Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies
PLoS ONE, 2011 The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health.
P. Schloss, D. Gevers, Sarah L. Westcott
semanticscholar +1 more source
16S classifier: a tool for fast and accurate taxonomic classification of 16S rRNA hypervariable regions in metagenomic datasets. [PDF]
PLoS ONE, 2015 The diversity of microbial species in a metagenomic study is commonly assessed using 16S rRNA gene sequencing. With the rapid developments in genome sequencing technologies, the focus has shifted towards the sequencing of hypervariable regions of 16S ...
Nikhil Chaudhary +4 more
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