Results 51 to 60 of about 417,138 (346)
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
Nature Communications, 2019 The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect.
Jethro S. Johnson +11 more
semanticscholar +1 more source
16S rRNA Methylase–producing, Gram-Negative Pathogens, Japan
Emerging Infectious Diseases, 2007 To investigate the exact isolation frequency of 16S rRNA methylase–producing, gram-negative pathogenic bacteria, we tested 87,626 clinical isolates from 169 hospitals. Twenty-six strains from 16 hospitals harbored 16S rRNA methylase genes, which suggests
Kunikazu Yamane +11 more
doaj +1 more source
Heliyon, 2022 This study aims to taxonomically identify and characterise the phylogenetic relationships of spiny lobsters based on mitochondrial cytochrome c oxidase I (COI) and 16S rRNA genes from Bangladesh waters.
Md. Sagir Ahmed +5 more
doaj +1 more source
Microbiome, 2014 Experimental designs that take advantage of high-throughput sequencing to generate datasets include RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), sequencing of 16S rRNA gene fragments, metagenomic analysis and selective ...
A. Fernandes +5 more
semanticscholar +1 more source
Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing
PLoS ONE, 2020 Microbial amplicon sequencing studies are an important tool in biological and biomedical research. Widespread 16S rRNA gene microbial surveys have shed light on the structure of many ecosystems inhabited by bacteria, including the human body.
A. Prodan +5 more
semanticscholar +1 more source
Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe [PDF]
Applied and Environmental Microbiology, 1989 16S rRNA from seven different Vibrio anguillarum strains was partially sequenced and compared. From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification of V. anguillarum. This was determined by RNA-DNA colony hybridization and slot-blot hybridization.
Åke Hagström +3 more
openaire +3 more sources
Clinical usefulness of 16S ribosomal RNA real-time PCR for the diagnosis of scrub typhus
Scientific Reports, 2021 Scrub typhus is a major acute febrile disease in the Asia–Pacific region. The purpose of the present study is to investigate the clinical usefulness of real-time PCR (Q-PCR) of 16S rRNA for the diagnosis of scrub typhus.
Na Ra Yun +4 more
doaj +1 more source
Microbiology Spectrum, 2022 Accurate identification of Elizabethkingia species mostly requires the use of molecular techniques, and 16S rRNA gene sequencing is generally considered the method of choice.
Jiun-Nong Lin +7 more
doaj +1 more source
ampvis2: an R package to analyse and visualise 16S rRNA amplicon data
bioRxiv, 2018 Summary Microbial community analysis using 16S rRNA gene amplicon sequencing is the backbone of many microbial ecology studies. Several approaches and pipelines exist for processing the raw data generated through DNA sequencing and convert the data into ...
K. S. Andersen +3 more
semanticscholar +1 more source