Results 71 to 80 of about 253,664 (304)

RRP9 Promotes Esophageal Squamous Cell Carcinoma Progression through E2F1 Transcriptional Regulation of CDK1

Advanced Biology, EarlyView.
The study reveals that RRP9 is abnormally highly expressed in ESCC tissues and is closely associated with poor prognosis in patients. Furthermore, it is found that RRP9 promotes ESCC progression through enhancing the E2F1‐mediated transcriptional regulation of CDK1.
Gang He, Yilong Wan, Yanbo Zhu, Bo Peng, Shengxiang Shao, Xinyi Zou, Zhenyu Han, Jiaxi Li, Sheng Ju, Xin Tong, Jun Zhao, Hansi Liang, Liuqing Zhang, Jiashi Xiong, Dong Jiang   +14 more
wiley   +1 more source

Rapid and reliable species-level identification from clinical samples using 16S rRNA gene nanopore sequencing analysis

Scientific Reports
The detection and identification of bacterial species in clinical samples are crucial for patient management and antibiotic treatment. When culture-based identification methods fail, 16S rRNA gene next-generation sequencing (NGS) serves as a valuable ...
Marco J. R. Ivens, Sergio Kamminga, Kawtar Benchamach, Chaimae Akile, Els Wessels, Eric C. J. Claas, Stefan A. Boers   +6 more
doaj   +1 more source

Limitations of 16S rRNA Gene Sequencing to Characterize Lactobacillus Species in the Upper Genital Tract

Frontiers in Cell and Developmental Biology, 2021
The endometrial cavity is an upper genital tract site previously thought as sterile, however, advances in culture-independent, next-generation sequencing technology have revealed that this low-biomass site harbors a rich microbial community which ...
Jessica L. O’Callaghan, Jessica L. O’Callaghan, Dana Willner, Dana Willner, Melissa Buttini, Flavia Huygens, Flavia Huygens, Elise S. Pelzer, Elise S. Pelzer   +8 more
doaj   +1 more source

A 16S rRNA gene sequencing and analysis protocol for the Illumina MiniSeq platform

bioRxiv, 2017
High-throughput sequencing of the 16S rRNA gene is widely used in microbial ecology, with Illumina platforms being widely used in recent studies. The MiniSeq, Illumina’s latest benchtop sequencer, enables more cost-efficient DNA sequencing relative to ...
M. Pichler, Ö. Coskun, Ana-Sofia Ortega-Arbulú, N. Conci, G. Wörheide, S. Vargas, W. Orsi   +6 more
semanticscholar   +1 more source

Consolidate Overview of Ribonucleic Acid Molecular Dynamics: From Molecular Movements to Material Innovations

Advanced Engineering Materials, EarlyView.
Molecular dynamics simulations are advancing the study of ribonucleic acid (RNA) and RNA‐conjugated molecules. These developments include improvements in force fields, long‐timescale dynamics, and coarse‐grained models, addressing limitations and refining methods.
Kanchan Yadav, Iksoo Jang, Jong Bum Lee
wiley   +1 more source

The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

PLoS ONE, 2014
Introduction Determining bacterial community structure in fecal samples through DNA sequencing is an important facet of intestinal health research. The impact of different commercially available DNA extraction kits upon bacterial community structures has
N. Kennedy, A. Walker, S. Berry, S. Duncan, F. Farquarson, P. Louis, J. Thomson, T CA JC H C A C P M CA JC JC CG C WG NJ A P K K DC Ahmad Anderson Barrett Drummond Edwards Hart Hawke, T. Ahmad, CA Anderson, JC Barrett, H. Drummond, C. Edwards, A. Hart, C. Hawkey, P. Henderson, M. Khan, C. Lamb, JC Lee, J. Mansfield, C. Mathew, C. Mowat, WG Newman, N. Prescott, A. Simmons, P. Simpson, K. Taylor, K. Taylor, D. Wilson, J. Satsangi, H. Flint, J. Parkhill, C. Lees, G. Hold   +33 more
semanticscholar   +1 more source

Photothermal Hydrogel with Mn3O4 Nanoparticles Alleviates Intervertebral Disc Degeneration by Scavenging ROS and Regulating Extracellular Matrix Metabolism

Advanced Functional Materials, EarlyView.
The MPTT‐nanozyme‐hydrogel system (Mn3O4@ChS‐HA) provides a multifunctional therapeutic strategy for intervertebral disc degeneration (IVDD), effectively targeting oxidative stress and enhancing AF repair by restoring extracellular matrix (ECM) and redox homeostasis.
Yangyang Chen, Chan Xue, Feifei Ni, Binwu Hu, Miaoheng Yan, He Zhang, Yuxiang Hu, Xiaoyao Peng, Guangfang Li, Yu Han, Hongjian Liu, Zengwu Shao, Qin Wang, Yulong Wei   +13 more
wiley   +1 more source

Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads: evaluation of effective study designs. [PDF]

PLoS ONE, 2013
Massively parallel high throughput sequencing technologies allow us to interrogate the microbial composition of biological samples at unprecedented resolution.
Orna Mizrahi-Man, Emily R Davenport, Yoav Gilad   +2 more
doaj   +1 more source

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing.

Journal of Visualized Experiments, 2019
The human gut is colonized by trillions of bacteria that support physiologic functions such as food metabolism, energy harvesting, and regulation of the immune system.
S. Shahi, K. Zarei, N. Guseva, A. Mangalam   +3 more
semanticscholar   +1 more source

Microneedle‐Based Biofertilizer Delivery Improves Plant Growth Through Microbiome Engineering

Advanced Functional Materials, EarlyView.
This figure shows how microneedles are used to deliver biofertilizers to enhance plant growth through microbial migration, metabolic reprogramming, and changes in plant endogenous microbiome. Abstract This study presents a microneedle‐based system for the delivery of rhizospheric biofertilizers into plant tissues to enhance growth.
Zhicheng Le, Sevugan Mayalagu, Cheng‐Yen Chen, Raktim Bhattacharya, Abhishek Gupta, Jingyuan Li, Zhi Wei Norman Teo, Yufeng Shou, Arya Gopinath MP, Danni Zhong, Tedrick Thomas Salim Lew, Hao Yu, Naweed I. Naqvi, Sanjay Swarup, Andy Tay   +14 more
wiley   +1 more source