Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis [PDF]
Molecules, 2018 (3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform.
Yuanzhi He +10 more
doaj +3 more sources
Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase [PDF]
Microbial Cell Factories, 2023 3-Hydroxybutanone (Acetoin, AC) and 2,3-butanediol (BD) are two essential four-carbon platform compounds with numerous pharmaceutical and chemical synthesis applications.
Yuchen Li +7 more
doaj +2 more sources
Engineering of Corynebacterium glutamicum for the enhanced production of optically pure (meso)-2,3-butanediol [PDF]
Bioresources and Bioprocessing2,3-Butanediol (2,3-BDO) is a versatile platform chemical with diverse applications in cosmetics, pharmaceuticals, agricultural and food manufacturing.
Eun Seo Song +3 more
doaj +2 more sources
Efficient 2,3-Butanediol Production from Ethanol by a Modified Four-Enzyme Synthetic Biosystem [PDF]
Molecules2,3-butanediol (2,3-BD) is a versatile bio-based platform chemical. An artificial four-enzyme synthetic biosystem composed of ethanol dehydrogenase, NADH oxidase, formolase and 2,3-butanediol dehydrogenase was designed for upgrading ethanol to 2,3-BD in ...
Jiming Zhang +6 more
doaj +2 more sources
Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W [PDF]
Biotechnology for Biofuels, 2020 Background Acetate is an abundant carbon source and its use as an alternative feedstock has great potential for the production of fuel and platform chemicals. Acetoin and 2,3-butanediol represent two of these potential platform chemicals. Results The aim
Katharina Novak +2 more
doaj +2 more sources
Protein Language Model-Guided Engineering of a 2,3-Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α-Hydroxy Ketones. [PDF]
Adv Sci (Weinh)A (2R,3R)‐butanediol dehydrogenase from Bacillus subtilis (BsBDH) is engineered for the enantioselective synthesis of 2‐hydroxycyclohexanone. A PASS computational design strategy is proposed to enhance the thermostability of BsBDH. Moreover, ESM‐1v combined with ISM is utilized for enhancing and inverting its stereoselectivity.
Ding H +5 more
europepmc +2 more sources
Frontiers in Microbiology, 2022 Brewers’ spent grains (BSG) are a by-product of the brewing industry that is mainly used as feedstock; otherwise, it has to be disposed according to regulations.
Blanka Didak Ljubas +9 more
doaj +1 more source
Effect of Botrytis cinerea Activity on Glycol Composition and Concentration in Wines
Fermentation, 2023 The content of 2,3-butanediol ((R,R) and meso isomers) and 1,2-propanediol in grape berries and “liquid samples” (all non-berry extracts) from the Tokaj wine region of Hungary was investigated. Our aim was to find out how the activity of Botrytis cinerea
Eszter Antal +3 more
doaj +1 more source
Production of methyl ethyl ketone from biomass using a hybrid biochemical/catalytic approach [PDF]
, 2013 The recent demand for sustainable routes to fuels and chemicals has led to an increased amount of research in conversion of natural resources. A potential approach for conversion of biomass to fuels and chemicals is to combine biochemical and chemical ...
Hohn, Keith L. +3 more
core +2 more sources
Two-stage pH control strategy based on the pH preference of acetoin reductase regulates acetoin and 2,3-butanediol distribution in Bacillus subtilis. [PDF]
PLoS ONE, 2014 Acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), which catalyzes the interconversion between acetoin and 2,3-butanediol, plays an important role in distribution of the products pools.
Xian Zhang +6 more
doaj +1 more source