Results 11 to 20 of about 139,462,453 (100)

The return of metabolism: biochemistry and physiology of glycolysis. [PDF]

Biol Rev Camb Philos Soc
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Grüning NM, Agostini F, Caldana C, Hartl J, Heinemann M, Keller MA, Krüsemann JL, Lamperti C, Linster CL, Lindner SN, Muenzner J, Nielsen J, Nikoloski Z, Siebers B, Snoep JL, Tenenboim H, Teusink B, Williams SJ, Wamelink MMC, Ralser M.   +19 more
europepmc   +2 more sources

GnuR Represses the Expression of Glucose and Gluconate Catabolism in Pseudomonas putida KT2440. [PDF]

Microb Biotechnol
In this work, we found that both glucose and gluconate can significantly induce the expression of catabolic enzymes of glucose and gluconate metabolism and the four related transcription factors including GnuR in Pseudomonas putida KT2440, a prime chassis for biotechnology.
Chen W, Ma R, Feng Y, Xiao Y, Sekowska A, Danchin A, You C.   +6 more
europepmc   +2 more sources

A Novel Lineage of Endosymbiotic Actinomycetales: Genome Reduction and Acquisition of New Functions in Bifidobacteriaceae Associated With Termite Gut Flagellates. [PDF]

Environ Microbiol
We have characterised several novel genera of Bifidobacteriaceae that colonise the cytoplasm of cellulolytic flagellates. Horizontal gene transfer played an important role in their adaptation to the intracellular environment. However, progressive genome erosion led to the loss of biosynthetic capacities that formed the basis of an originally ...
Silva JK, Hervé V, Mies US, Platt K, Brune A.   +4 more
europepmc   +2 more sources

Sustainable biosynthesis of chemicals from methane and glycerol via reconstruction of multi‐carbon utilizing pathway in obligate methanotrophic bacteria

Microbial Biotechnology, Volume 14, Issue 6, Page 2552-2565, November 2021., 2021
To address the limitation of native C1 metabolism of obligate type I methanotrophs, we proposed a novel platform strain that can utilize methane and multi‐carbon substrates, such as glycerol, simultaneously to boost growth rates and chemical production in Methylotuvimicrobium alcaliphilum 20Z. To demonstrate the uses of this concept, we reconstructed a
Hoa Thi Quynh Le, Anh Duc Nguyen, Ye Rim Park, Eun Yeol Lee   +3 more
wiley   +1 more source

Pectobacterium atrosepticum KDPG aldolase, Eda, participates in the Entner–Doudoroff pathway and independently inhibits expression of virulence determinants

Molecular Plant Pathology, Volume 22, Issue 2, Page 271-283, February 2021., 2021
A component of the Entner–Doudoroff pathway, 2‐keto‐3‐deoxy‐6‐phosphogluconate aldolase, plays a vital role in the virulence of Pectobacterium atrosepticum on potato by controlling expression of genes involved in pectin degradation. Abstract Pectobacterium carotovorum has an incomplete Entner–Doudoroff (ED) pathway, including enzyme 2‐keto‐3‐deoxy‐6 ...
Huan Wang, Yujie Wang, Sonia Humphris, Weihua Nie, Pengfei Zhang, Frank Wright, Emma Campbell, Baishi Hu, Jiaqin Fan, Ian Toth   +9 more
wiley   +1 more source

Altering the substrate specificity of 2-keto-3-deoxy-6-phosphogluconate aldolase by rational mutagenesis and directed evolution.

, 2007
The need to synthesize enantiomerically pure pharmaceutical, agrochemical, and food agents, has prompted our efforts to develop stereospecific aldol catalysts using 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase.
Cheriyan, Manoj
core   +5 more sources

Insights into the Substrate Specificity of Archaeal Entner–Doudoroff Aldolases: The Structures of Picrophilus torridus 2‑Keto-3-deoxygluconate Aldolase and Sulfolobus solfataricus 2‑Keto-3-deoxy-6-phosphogluconate Aldolase in Complex with 2‑Keto-3-deoxy-6-phosphogluconate [PDF]

, 2018
The thermoacidophilic archaea Picrophilus torridus and Sulfolobus solfataricus catabolize glucose via a nonphosphorylative Entner–Doudoroff pathway and a branched Entner–Doudoroff pathway, respectively. Key enzymes for these Entner–Doudoroff pathways are
Garry L. Taylor (2230780), Peter Schönheit, Reher, Matthias, Matthias Reher, Matthias Reher (5388788), Viatcheslav Zaitsev, Peter Schönheit (1919461), Ortjohann, Marius, Susan J. Crennell, Michael J. Danson (516069), Ulrike Johnsen (1919455), Marius Ortjohann (5388785), Viatcheslav Zaitsev (5388791), Garry L. Taylor, Ulrike Johnsen, Michael J. Danson, Zaitsev, Viatcheslav, Schönheit, Peter, Marius Ortjohann, Susan J. Crennell (2272984), Johnsen, Ulrike, Taylor, Garry, Danson, Michael; id_orcid, Crennell, Susan; id_orcid   +23 more
core   +1 more source

Engineering Pseudomonas putida KT2440 for the production of isobutanol

Engineering in Life Sciences, Volume 20, Issue 5-6, Page 148-159, April 2020., 2020
Abstract We engineered P. putida for the production of isobutanol from glucose by preventing product and precursor degradation, inactivation of the soluble transhydrogenase SthA, overexpression of the native ilvC and ilvD genes, and implementation of the feedback‐resistant acetolactate synthase AlsS from Bacillus subtilis, ketoacid decarboxylase KivD ...
Robert Nitschel, Andreas Ankenbauer, Ilona Welsch, Nicolas T. Wirth, Christoph Massner, Naveed Ahmad, Stephen McColm, Frédéric Borges, Ian Fotheringham, Ralf Takors, Bastian Blombach   +10 more
wiley   +1 more source

Data_Sheet_1_Enzymatic Synthesis of 2-Keto-3-Deoxy-6-Phosphogluconate by the 6-Phosphogluconate-Dehydratase From Caulobacter crescentus.pdf

, 2020
The availability of metabolic intermediates is a prerequisite in many fields ranging from basic research, to biotechnological and biomedical applications as well as diagnostics.
Roland Meier (65695), Bettina Siebers (81513), Rudolf Koehling (8603136), Eric Allenspach (8603139), Timon Bohnen (8603130), Bernhard Schoenenberger (2661361), Klara Bangert (8603133), Sabine Krevet (8603127), Christopher Bräsen (406182), Markus Obkircher (2025316), Lu Shen (161789)   +10 more
core   +1 more source

Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase [PDF]

, 2009
Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase (SacKdgA) displays optimal activity at 95 degrees C and is studied as a model enzyme for aldol condensation reactions.
Siemerink, M.A.J., Kengen, S.W.M., Kaper, T., Oost, J., van der, Perrakis, G., Wolterink-van Loo, S.   +5 more
core   +1 more source