Results 1 to 10 of about 254 (79)

Microbial succession in human tissues postmortem: insights from 2bRAD-M sequencing [PDF]

Microbiology Spectrum
Microbial communities play a crucial role in decomposition, yet their patterns in human tissues remain underexplored. Most previous research has often focused on animal models such as mice and swine, with limited studies on human samples, primarily ...
Xin Huang, Jianye Zeng, Fan Yang, Yanan Liu, Ji Chen, Hongyan Wang, Shilin Li, Chengtao Li, Suhua Zhang   +8 more
doaj   +7 more sources

Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M [PDF]

Genome Biology, 2022
Microbiome samples with low microbial biomass or severe DNA degradation remain challenging for amplicon-based or whole-metagenome sequencing approaches. Here, we introduce 2bRAD-M, a highly reduced and cost-effective strategy which only sequences ~ 1% of
Zheng Sun, Shi Huang, Pengfei Zhu, Lam Tzehau, Helen Zhao, Jia Lv, Rongchao Zhang, Lisha Zhou, Qianya Niu, Xiuping Wang, Meng Zhang, Gongchao Jing, Zhenmin Bao, Jiquan Liu, Shi Wang, Jian Xu   +15 more
doaj   +4 more sources

16S rRNA, metagenomics and 2bRAD-M sequencing to decode human thanatomicrobiome [PDF]

Scientific Data
Microorganisms are essential in the decomposition of corpses and play a significant role in forensic science. However, previous studies have primarily focused on animal remains, specifically the gut, skin, and burial environment.
Xin Huang, Jianye Zeng, Shilin Li, Ji Chen, Hongyan Wang, Chengtao Li, Suhua Zhang   +6 more
doaj   +4 more sources

High-resolution microbiome analysis of host-rich samples using 2bRAD-M without host depletion [PDF]

npj Biofilms and Microbiomes
Characterizing human microbiota in host-dominated samples is crucial for understanding host-microbe interactions, yet is challenged by the high host DNA context (HoC). Current depletion strategies are limited by DNA loss and require immediate processing.
Yuesong Jiang, Jiang Liu, Yufeng Zhang, Lisha Zhou, Enoch Kao, Shuwen Hou, Qianya Niu, Yongxin Liu, Zhenjiang Zech Xu, Tao Ding, Yu-Xiong Su, Yu Liu, Gao Zhang, Xiuping Wang, Fei Teng, Shi Huang   +15 more
doaj   +3 more sources

Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M [PDF]

Frontiers in Microbiology
IntroductionThe microbial composition of human breast milk and infant meconium offers critical insights into the early microbial colonization profile, and it greatly contributes to the infant’s immune system and long-term health outcomes.
Shuwen Hou, Yuesong Jiang, Feng Zhang, Tianfan Cheng, Dan Zhao, Jilong Yao, Ping Wen, Ping Wen, Lijian Jin, Shi Huang   +9 more
doaj   +4 more sources

Characterization of pathogenic microbiome on removable prostheses with different levels of cleanliness using 2bRAD-M metagenomic sequencing

Journal of Oral Microbiology
Background The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology.Objectives To characterize the microbiome of removable prostheses with different levels of ...
Tong Wah Lim, Shi Huang, Yuesong Jiang, Yufeng Zhang, Michael Francis Burrow, Colman McGrath   +5 more
doaj   +5 more sources

Urobiome of patients with diabetic kidney disease in different stages is revealed by 2bRAD-M [PDF]

Journal of Translational Medicine
Background Knowledge of the urinary microbiome (urobiome) in diabetic kidney disease (DKD) remains limited. The most commonly used 16S rRNA sequencing technique can only provide bacterial identification at the genus level.
Nan Li, Ying Wang, Haojun Zhang, Liang Ma, Xinhui Huang, Yuhang Qiao, Han Li, Hailing Zhao, Ping Li   +8 more
doaj   +3 more sources

Vaginal microbiota in term pregnant women with differences in cervical ripeness revealed by 2bRAD-M [PDF]

BMC Microbiology
Background Cervical ripening is a multifactorial outcome, and the association between cervical ripening and vaginal microbiota remains unexplored in term primiparous women.
Saihu Lu, Qi Wu, Wenzhu He, Xue Du, Qianqian Cui, Yuanyuan Yang, Zongzhi Yin   +6 more
doaj   +3 more sources

Identification of intestinal microbial population structure of Penaeus vannamei supplemented with chitosan oligosaccharide using 2bRAD-M [PDF]

Microbiology Spectrum
The intestinal microbiota of Penaeus vannamei is critical for its health and disease resistance, but imbalances can increase the risk of infections, especially by pathogenic Vibrio species.
Chunpeng Fu, Xiaopeng Fu, Zongzhen Li, Chengjiang Xu, Fajun Li, LiXin Wang, Lifang Wang   +6 more
doaj   +4 more sources

2bRAD-M reveals differences in microbial communities between Modic changes and disc herniation [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundModic changes are caused by various factors, such as degenerative processes, inflammation, biomechanical, genetic, and metabolic factors, infection, and smoking. Bacteria have been identified in human intervertebral discs by 16S rRNA sequencing;
Fang Yang, Jingtao Zhang, Junpu Zha, Guolei Zhang, Jia Li, Wei Du, Lin Liu, Jun Di   +7 more
doaj   +4 more sources