pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Data in Brief, 2015 Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by
Marina Hanneken +2 more
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Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
Biological Procedures Online, 2009 The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Mandal Nakul +4 more
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Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature
BMC Plant Biology, 2023 Background Low temperature (LT) stress is one of the major environmental stress factors affecting the growth and yield of maize (Zea mays L.). Hence, it is important to unravel the molecular mechanisms behind LT stress tolerance to improve molecular ...
Salika Ramazan, Nelofer Jan, Riffat John
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Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins
Bioscience, Biotechnology, and Biochemistry, 2002 Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal ...
MIKAMI, Satoshi +3 more
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BMC Genomics, 2004 Background Two-dimensional gel electrophoresis (2D-PAGE) has proven over the years to be a reliable and efficient method for separation of hundreds of proteins based on charge and mass.
Kashani-Poor Noushin +7 more
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Direct Versus Indirect Measurement of Digit Ratio (2D:4D): A Critical Review of the Literature and New Data [PDF]
, 2016 Digit ratio (2D:4D: the relative lengths of the second and fourth digits) is widely used as a correlate of prenatal sex steroids. There are two common methods of measuring 2D:4D, the direct method and the indirect method.
Manning, John +7 more
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Object-based 2D-to-3D video conversion for effective stereoscopic content generation in 3D-TV applications [PDF]
, 2011 Three-dimensional television (3D-TV) has gained increasing popularity in the broadcasting domain, as it enables enhanced viewing experiences in comparison to conventional two-dimensional (2D) TV.
Feng, Yue, Ren, Jinchang, Jiang, Jianmin
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Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE
Bio-Protocol, 2018 The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system.
Marjaana Rantala +2 more
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Influence of extraction method on protein profile of soybeans [PDF]
Hemijska Industrija, 2013 Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone ...
Pavlićević Milica Ž. +2 more
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Effect of Heat Treatment on the Property, Structure, and Aggregation of Skim Milk Proteins
Frontiers in Nutrition, 2021 To study the mechanism of heat-induced protein aggregates, skim milk was heated at 55, 65, 75, 85, and 95°C for 30 s. Then, the sulfhydryl content, surface hydrophobicity, and secondary structure of heat-treated skim milk were studied. Treating skim milk
Hongbo Li +4 more
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