Results 71 to 80 of about 5,167,336 (292)
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
Indication on the 2D-PAGE of the immunoreactive spots identified in 2D-WB experiments.
, 2014 Adult worm total (AW-TOT) and tegumental (AW-TEG) protein extracts were separated by 2-DE using 7 cm pH 3–10 IPG strips followed by SDS-PAGE 12%, and stained by Colloidal Coomassie Blue G-250. Protein spots immunoreactive to INF, NE and/or NI pooled sera
Andrea Gazzinelli (169153) +8 more
core +1 more source
Regional profiling reveals a distinct glioblastoma infiltrative margin proteome
Scientific ReportsIsocitrate dehydrogenase wild-type glioblastoma, a malignant brain tumour of glial origin, confers a poor prognosis with a median survival of 12 to 16 months from diagnosis.
Artur Kocon +5 more
doaj +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry
Biological Procedures Online, 2010 The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Honoré Bent +4 more
doaj
Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source
Molecular Oncology, EarlyView.Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq +8 more
wiley +1 more source
, 2014 A) 2D-PAGE of adult worm total (AW-TOT) and tegumental (AW-TEG) protein extracts using 7 cm, pH 3–10, 3–10NL and 5–8 IPG strips and SDS-PAGE 12%, stained by Colloidal Coomassie Blue G-250. B) Corresponding 2D-WB using pool of S.
Andrea Gazzinelli (169153) +8 more
core +1 more source
Molecular Oncology, EarlyView.LUNAR is a liver‐specific long noncoding RNA (lncRNA) that is highly expressed in normal liver but becomes epigenetically silenced in hepatocellular carcinoma through promoter hypermethylation. Loss of LUNAR is associated with NOTCH activation, epithelial–mesenchymal transition, and metastasis, whereas restoring LUNAR restrains metastatic progression ...
Se Ha Jang +9 more
wiley +1 more source
Representative 2D-PAGE image of each study group including control, CIS, RRMS and PMS.
, 2015 Representative 2D-PAGE image of each study group including control, CIS, RRMS and PMS.
O. Uğur Sezerman (735106) +8 more
core +1 more source