Results 111 to 120 of about 5,167,436 (292)

Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species

Toxins, 2017
Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators.
Nezahat Pınar Barkan, Mustafa Bilal Bayazit, Duygu Ozel Demiralp   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Assessment criteria for 2D shape transformations in animation [PDF]

, 1997
The assessment of 2D shape transformations (or morphing) for animation is a difficult task because it is a multi-dimensional problem. Existing morphing techniques pay most attention to shape information interactive control and mathematical simplicity ...
Patterson, J., Yu, J-H., J.W. Patterson, Jinhui Yu   +3 more
core   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Protein separation, staining and comparison determined by 2D-DEGE.

, 2013
The protein extracts from hippocampus of the control group and 10.75 mg/kg groups were profiled using 2D-DIGE analysis as described in the Materials and methods.
Fan Hu (277859), Yubang Wang (277857), Guixiang Ji (240455), Yan Long (118326), Aihua Gu (240457), Lifeng Yan (277858)   +5 more
core   +1 more source

Hawking-Page and entanglement phase transition in 2d CFT on curved backgrounds

Journal of High Energy Physics
The thermodynamics and the entanglement properties of two-dimensional conformal field theories (2d CFTs) on curved backgrounds are studied. By means of conformal mapping we study the equivalent system on flat space governed by the deformed Hamiltonian ...
Akihiro Miyata, Masahiro Nozaki, Kotaro Tamaoka, Masataka Watanabe   +3 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Proteins differently synthesized between HT29 and SW480 cells as determined by 2D-PAGE fluorescence.

, 2014
Checkmarks indicate correlation with shotgun analysis (SG).Proteins differently synthesized between HT29 and SW480 cells as determined by 2D-PAGE fluorescence.
David M. Baron (468294), Christopher Gerner (117547), Brigitte Marian (420584), Editha Bayer (673205), Verena J. Haudek-Prinz (673204), Clemens Röhrl (503498), Ulrike Kaindl (673203)   +6 more
core   +1 more source