Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
On $Ω_{3c}NN$ and $Ω_{3c} Ω_{3c} N$ systems with HAL QCD potentials
This study employs the Faddeev formalism in configuration space to investigate the $Ω_{3c}NN$ cluster containing a triply charmed Omega baryon ($Ω_{3c}$). Using the recently reported HAL QCD $S$-wave $Ω_{3c}N$ potentials in the $^3S_1$ and $^5S_2$ channels, together with the MT-I--III nucleon--nucleon potential and neglecting the Coulomb force, we find
Filikhin, Igor +2 more
openaire +2 more sources
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
3C channel business model and management strategy in small and medium sized enterprise
, 2016 碩士因應近年來電子商務發展衝擊實體3C通路經營，加上消費性電子產品的發展日新月異，實體3C通路如何面對產品結構改變，其他型態的通路競爭，這些問題考驗著實體3C通路資源是否能有効分配。本研究以全台最大型的3C實體通路「燦坤3C」、最大家電連鎖通路「全國電子」、全國唯一的招商式3C通路「NOVA資訊廣場」與「順發3C」及日本實體3C通路發展概況，並以個案研究法、資源分配理論，依據Eisenhardt(1989)提出的個案研究，以燦坤3C、全國電子及NOVA資訊廣場與日本實體3C通路發展概況為探討對象 ...
唐瑞昇;Tang, Jui-Sheng
core
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Faraday rotation and depolarization in the powerful DRAGNs 3C 34, 3C 228 and 3C 340 [PDF]
Monthly Notices of the Royal Astronomical Society, 1995 R. A. Johnson +2 more
openaire +1 more source
Integrating Mathematics and Digital Technologies: A practical teaching approach using the 3C Model
In this article, the authors explore how the 3C Model can be used to integrate curriculum areas with mathematics, namely digital technologies.
Martin, David A. +2 more
core
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
FEBS Open Bio, EarlyView.Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source
New Observations of 3C 382, 3C 452 and 3C 465 at 2.7 and 5 GHz [PDF]
Monthly Notices of the Royal Astronomical Society, 1973 J. M. Riley, N. J. B. A. Branson
openaire +1 more source