Results 91 to 100 of about 22,635,692 (264)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
The technology clubs: the distribution of knowledge across nations [PDF]
The convergence clubs literature in applied growth theory suggests that countries that differ in terms of structural characteristics and initial conditions tend to experience diverging growth performances.
Castellacci, Fulvio
core
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
An impact model for evaluating 4-H programs for the handicapped child
, 1980 The purpose of the study was to (1) determine the number of handicapped children being served by 4-H programs of the Indiana Cooperative Extension Service, (2) describe the organization and the presentation of the programs, and (3) develop a model for ...
Branson, Floyd
core
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Approximate cores of games and economies with clubs [PDF]
We introduce the framework of parameterized collections of games and provide three nonemptiness of approximate core theorems for arbitrary games with and without sidepayments.
Alexander Kovalenkov, Myrna H. Wooders
core
Charter Application for 4-H Clubs and Cloverbud Groups
, 2020 To apply for a 4-H Charter, the organizational leader of a 4-H club or Cloverbud group should submit one copy of this application to the local Extension office if the club or group is based at a county or city level.
Proudfoot, Chad N.
core
Virginia 4-H Foundation Newsletter
, 2014 The Virginia 4-H Foundation newsletter contains highlights of recent impacts and successes related to philanthropic support of Virginia 4-H. Volume II, Issue I includes a message from the chair, as well as pieces on the Virginia 4-H Youth Livestock ...
Grove, Benjamin +2 more
core +1 more source