Results 151 to 160 of about 12,456,978 (213)
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Calf intestinal 5′-nucleotidase
Archives of Biochemistry and Biophysics, 1966Abstract The 5′-nucleotidase activity isolated from calf intestinal mucosa has been resolved into three fractions by DEAE-cellulose column chromatography. One of these fractions was obtained free of nonspecific phosphatase activity and was used for further studies.
Melvin S. Center, Francis J. Behal
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5′-Nucleotidase Activities in Human Fetus
Neonatology, 1985Specific 5′-nucleotidase (5′-N) activity in 17 human fetuses was systemically studied in order to get basic values of the enzyme activity. A wide spectrum of 5′-N activity among the organs was revealed. Very strong activity was found in placenta (42.62 ± 19.24 nmol adenosine/mg protein/min), skeletal muscle (31.80 ± 11.10) and skin (27.31 ± 13.00 ...
S, Amano +5 more
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Diadenosine tetraphosphate activates cytosol 5′-nucleotidase
Biochemical and Biophysical Research Communications, 1986The rate of hydrolysis of IMP (0.5 mM) by cytosol 5'-nucleotidase from Artemia embryos was increased up to 7-fold by concentrations of around 10 microM diadenosine tetraphosphate (Ap4A). Half maximal activation of the enzyme was accomplished with 5 microM Ap4A.
R M, Pinto +3 more
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5-Nucleotidase activity in lymphocytes
The Histochemical Journal, 1981Some characteristics of lymphocyte 5'-nucleotidase are reviewed. The optimal conditions for the cytochemical localization of 5'-nucleotidase (AMPase) in the mouse lymphocyte have been established. Quantitative monitoring of the effects of fixation and the components of the cytochemical medium showed that cytochemistry can be performed under conditions ...
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Detergent solubilization of cardiac 5′-nucleotidase
Experientia, 1983Nonionic, anionic and zwitterionic detergents were employed for solubilization of 5′-nucleotidase from acetone powder preparations of rat heart. Zwittergents effectively solubilized the enzyme and may allow now its further identification and purification by electrophoretic techniques.
G, Lutoslawska, N, Jha, H P, Baer
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5′-Nucleotidase in Rat Liver Lysosomes
The Journal of Biochemistry, 19875'-Nucleotidase was found in purified rat liver tritosomes. When tritosomes were subfractionated into the membrane and soluble contents fractions, 73% of the total 5'-nucleotidase activity was found in the membrane fraction and 24% in the soluble contents fraction.
I, Wada, S, Eto, M, Himeno, K, Kato
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Ecto-Enzyme of Granulocytes: 5′-Nucleotidase
Science, 1974The 5′-nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. The active site of this enzyme faces the external medium, not the cytoplasm.
J W, DePierre, M L, Karnovsky
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