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Inter- and intra-strain variation in the 5.8S ribosomal RNA and internal transcribed spacer sequences of Entamoeba histolytica and comparison with Entamoeba dispar, Entamoeba moshkovskii and Entamoeba invadens1New sequence data reported here are deposited in GenBank with the following accession numbers: X89635, Y12249, Y12250 and Y12251.1

International Journal of Parasitology, 2000
Indrani Som, Amir Azam, Alok Bhattacharya, Sudha Bhattacharya   +3 more
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Alternative conformational states in the ribosome-associated 5.8S RNA

European Journal of Biochemistry, 1984
Two conformations of the 5.8S rRNA have been distinguished by gel electrophoresis. Diethyl pyrocarbonate and dimethyl sulphate reactivity were used to probe these conformational states in both the free molecule and those associated with 28S RNA or the ribosome.
A C, Lo, W, Liu, R N, Nazar
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The primary structure of lupin seed 5.8 S ribosomal RNA

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990
The lack of colinearity between nucleotide sequence of the lupin 5.8 S rDNA gene (Rafalski, A.J., Wiewiórowski, M. and Soll, D. (1983) FEBS Lett. 152, 241-246) and 5.8 S rRNA of other plants (Erdmann, V.A. and Wolters, J. (1986) Nucleic Acids Res. 14, r1-r59.) prompted us to clarify this point by sequencing the native lupin 5.8 S rRNA.
M Z, Barciszewska, T D, Mashkova, J, Barciszewski   +2 more
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Comparison of the Nucleotide Sequence and Secondary Structure of the 5.8S Ribosomal RNA Gene ofChlamydomonas tetragamawith those of Green Algae

DNA Sequence, 1998
Hidenobu Uchida, Kaori Kitae, Ken-Ich Tomizawa, Akiho Yokota   +3 more
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Effects of ribosome dissociation on the structure of the ribosome-associated 5.8S RNA

Biochemistry and Cell Biology, 1987
Diethyl pyrocarbonate reactivity and thermal denaturation were used to probe potential ribosomal interactions between tRNA and the small 5.8S and 5S rRNAs. Puromycin, an analogue of the terminal aminoacyl-adenosine portion of aminoacyl-tRNA, was observed to increase the accessibility of the 5.8S rRNA, including the highly conserved GAACp sequences ...
A C, Lo, W Y, Liu, D E, Culham, R N, Nazar   +3 more
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Structure of the ribosome-associated 5·8 S ribosomal RNA

Journal of Molecular Biology, 1983
The structure of the 5.8 S ribosomal RNA in rat liver ribosomes was probed by comparing dimethyl sulfate-reactive sites in whole ribosomes, 60 S subunits, the 5.8 S-28 S rRNA complex and the free 5.8 S rRNA under conditions of salt and temperature that permit protein synthesis in vitro.
Wangyi Liu, Amy C. Lo, Ross N. Nazar, M. Gellert   +3 more
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Nucleolar ribosomal RNA synthesis continues in differentiating lens fiber cells until abrupt nuclear degradation required for ocular lens transparency

RNA Biology
Cellular differentiation requires highly coordinated action of all three transcriptional systems to produce rRNAs, mRNAs and various ‘short’ and ‘long’ non-coding RNAs by RNA Polymerase I, II and III systems, respectively.
Danielle Rayêe, U. T. Meier, C. Eliscovich, A. Cvekl   +3 more
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Third Metal Ion Dictates the Catalytic Activity of the Two-Metal-Ion Pre-Ribosomal RNA-Processing Machinery.

Angewandte Chemie
Nucleic acid processing enzymes use a two-Mg2+-ion motif to promote the formation and cleavage of phosphodiester bonds. Yet, recent evidence demonstrates the presence of spatially conserved second-shell cations surrounding the catalytic architecture of ...
J. Borišek, Jana Aupič, A. Magistrato
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High resolution landscape of ribosomal RNA processing and surveillance

Nucleic Acids Research
Ribosomal RNAs are processed in a complex pathway. We profiled rRNA processing intermediates in yeast at single-molecule and single-nucleotide levels with circularization, targeted amplification and deep sequencing (CircTA-seq), gaining significant ...
Weidong An, Yunxiao Yan, K. Ye
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The ribosomal 5.8S RNA: eukaryotic adaptation or processing variant?

Canadian Journal of Biochemistry and Cell Biology, 1984
A striking difference between the cytoplasmic ribosomes of eukaryotes and prokaryotes is the presence (in eukaryotes) of one additional RNA component, the 5.8S rRNA. This RNA, which is hydrogen bonded to the cognate high molecular weight RNA of the large subunit, is about 160 nucleotides in length, and is cotranscribed with the high molecular weight ...
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