Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland
Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland.
Jakub Skorupski+4 more
doaj +1 more source
Dynamics of ribosomes in mRNA translation under steady and non-steady state conditions [PDF]
Recent advances in DNA sequencing and fluorescence imaging have made it possible to monitor the dynamics of ribosomes actively engaged in messenger RNA (mRNA) translation. Here, we model these experiments within the inhomogeneous totally asymmetric simple exclusion process (TASEP) using realistic kinetic parameters.
arxiv +1 more source
Interaction of 5-S RNA, 5.8-S RNA and tRNA with Rat-Liver Ribosomal Proteins [PDF]
In order to ascertain 5-S RNA, 5.8-S RNA and tRNA binding proteins from eukaryotic ribosomes, affinity chromatography of the rat liver ribosomal 40-S and 60-S subunit proteins on immobilized RNAs was used. Rat liver 5-S RNA, 5.8-S RNA and tRNA were immobilized via 3′-end ribose to adipic-acid-hydrazide-epoxy-activated Sepharose 6B.
Mart Ustav+4 more
openaire +3 more sources
Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
Objective Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. Results Key modifications
Katelyn M. Kimble+2 more
doaj +1 more source
Polyporales is a diverse group of Agaricomycetes including more than 2,500 species belonging to 255 genera and 18 families. Recently, many studies focused on the classification of Polyporales, but the familial placements of some taxa remain uncertain. In
Shun Liu+5 more
doaj +1 more source
Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination [PDF]
In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E.
arxiv +1 more source
Short-term sequence evolution and vertical inheritance of the
Background Ribosomal DNA of several species of the free-living Naegleria amoeba harbors an optional group I intron within the nuclear small subunit ribosomal RNA gene.
De Jonckheere Johan F+3 more
doaj +1 more source
Stochastic kinetics of ribosomes: single motor properties and collective behavior [PDF]
Synthesis of protein molecules in a cell are carried out by ribosomes. A ribosome can be regarded as a molecular motor which utilizes the input chemical energy to move on a messenger RNA (mRNA) track that also serves as a template for the polymerization of the corresponding protein.
arxiv +1 more source
rDNA Copy Number Variation and Methylation During Normal and Premature Aging
In > 160 healthy individuals the absolute rDNA copy number (CN) in blood ranged from approximately 240 to 900. The number of active copies with a completely unmethylated promoter varied from 40 to 120, independent of absolute CN. Promoter methylation is a compensation mechanism for absolute CN variation. Aging was associated with a loss of unmethylated
Alva B. C. Geisen+5 more
wiley +1 more source
Chicken rRNA Gene Cluster Structure. [PDF]
Ribosomal RNA (rRNA) genes, whose activity results in nucleolus formation, constitute an extremely important part of genome. Despite the extensive exploration into avian genomes, no complete description of avian rRNA gene primary structure has been ...
Alexander G Dyomin+7 more
doaj +1 more source