Resurrection of an ancestral 5S rRNA
Background In addition to providing phylogenetic relationships, tree making procedures such as parsimony and maximum likelihood can make specific predictions of actual historical sequences.
Fox George E, Lu Qing
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Cytogenetic characterisation of the razor shells "Ensis directus" (Conrad, 1843) and "E. minor" (Chenu, 1843) (Mollusca: Bivalvia) [PDF]
[Abstract] The European razor shell Ensis minor (Chenu 1843) and the American E. directus (Conrad 1843) have a diploid chromosome number of 38 and remarkable differences in their karyotypes: E.
Egea, Emilie+5 more
core +2 more sources
Comparison of transcriptional activity profiling by metabolic labeling or nuclear RNA sequencing
Significance Statement A uracil analog, EU, can be incorporated in vivo into newly synthesized RNA during normal transcription. In maize root tips, we found the entire population of nuclear RNA is remarkably similar in composition to newly synthesized RNA, but both differ substantially from cellular RNA, which is largely of cytoplasmic origin.
E. E. Wear+5 more
wiley +1 more source
In forensics, post-mortem interval (PMI) estimation is essential. Researchers have devised various approaches to accurately determine PMI, consequently, ribonucleic acid (RNA) molecules could be useful PMI estimation tool.
Onyekachi Ogbonnaya Iroanya+5 more
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Populations of seven Ancistrus species were analyzed from streams and rivers of three hydrographic Brazilian basins. All populations showed different diploid numbers (2n), fundamental numbers (FNs), and karyotypes.
Sandra Mariotto+4 more
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TRE5-A retrotransposition profiling reveals putative RNA polymerase III transcription complex binding sites on the Dictyostelium extrachromosomal rDNA element. [PDF]
The amoeba Dictyostelium discoideum has a haploid genome in which two thirds of the DNA encodes proteins. Consequently, the space available for selfish mobile elements to expand without excess damage to the host genome is limited.
Thomas Spaller+3 more
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Here, we report that mitochondria from rat skeletal muscle contain a select population of nuclear‐encoded protein‐coding and noncoding RNAs, including 3 microRNAs whose expression tended to be altered by exercise training. These findings suggest that mitochondria‐localized nuclear‐encoded RNAs may play a role in mediating the beneficial adaptive ...
Jessica L. Silver+8 more
wiley +1 more source
Long-term evolution of 5S ribosomal DNA seems to be driven by birth-and-death processes and selection in "Ensis" razor shells (Mollusca: Bivalvia) [PDF]
[Abstract] A study of nucleotide sequence variation of 5S ribosomal DNA from six Ensis species revealed that several 5S ribosomal DNA variants, based on differences in their nontranscribed spacers (NTS), occur in Ensis genomes.
González-Tizón, Ana M.+2 more
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Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
Objective Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. Results Key modifications
Katelyn M. Kimble+2 more
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Kinetic Studies on the Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in Intact Cells of Neurospora crassa [PDF]
The transport of cytoplasmically synthesized mitochondrial proteins was investigated in whole cells of Neurospora crassa, using dual labelling and immunological techniques.
Hallermayer, Gerhard+2 more
core +1 more source