Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
BMC Research Notes, 2018 Objective Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. Results Key modifications
Katelyn M. Kimble +2 more
doaj +1 more source
Glutathione reductase-catalyzed cascade of redox reactions to bioactivate potent antimalarial 1,4-naphthoquinones--a new strategy to combat malarial parasites. [PDF]
, 2011 Our work on targeting redox equilibria of malarial parasites propagating in red blood cells has led to the selection of six 1,4-naphthoquinones, which are active at nanomolar concentrations against the human pathogen Plasmodium falciparum in culture and ...
Bauer, Holger +12 more
core +2 more sources
Molecular Identification of the Nematode Eustrongylides ignotus in Mandarin Fish, Siniperca chuatsi
Aquaculture, Fish and Fisheries, Volume 5, Issue 6, December 2025.ABSTRACT Mandarin fish (Siniperca chuatsi) is a high‐value economic freshwater fish in China; however, the frequent occurrence of diseases threatens its production. To this end, we explored the impact of a nematode parasite newly found in the abdomen of mandarin fish collected from Pudong, Shanghai, China.
Jin‐hua Gao +8 more
wiley +1 more source
Viability of Self‐Taken Vaginal Swab Samples for RNA‐Based Biomarker Analysis in Cervical Disease
Journal of Medical Virology, Volume 97, Issue 12, December 2025.ABSTRACT High risk human papillomaviruses (hrHPVs) cause most cervical cancers. Cervical screening programmes aim to identify precancerous disease using detection of hrHPV nucleic acid using clinician‐taken liquid‐based cytology (LBC) samples, or increasingly, self‐taken samples (STSs).
Harry Scott +7 more
wiley +1 more source
BMC Plant Biology, 2010 Background In flowering plants and animals the most common ribosomal RNA genes (rDNA) organisation is that in which 35S (encoding 18S-5.8S-26S rRNA) and 5S genes are physically separated occupying different chromosomal loci.
Garcia Sònia +3 more
doaj +1 more source
Representation, searching and discovery of patterns of bases in complex RNA structures [PDF]
We describe a graph theoretic method designed to perform efficient searches for substructural patterns in nucleic acid structural coordinate databases using a simplified vectorial representation.
Artymiuk, P.J. +3 more
core +1 more source
Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses [PDF]
, 2017 Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to the high concentration of ...
Acevedo, Raúl Maximiliano +4 more
core +1 more source
Patterns and regulation of ribosomal RNA transcription in
BMC Microbiology, 2011 Background Borrelia burgdorferi contains one 16S and two tandem sets of 23S-5S ribosomal (r) RNA genes whose patterns of transcription and regulation are unknown but are likely to be critical for survival and persistence in its hosts. Results RT-PCR of B.
Schwartz Ira +3 more
doaj +1 more source
Molecular Genetics & Genomic Medicine, 2019 Background Ribosomal RNA (rRNA) consists of four non‐coding RNAs, the 28S, 5.8S, 18S, and 5S rRNA. Abnormal expression of rRNA has been found in multiple tumors, and the methylation of rDNA promoter may affect rRNA expression as an epigenetic regulatory ...
Shanshan Ha +4 more
doaj +1 more source
Conservation and loss of ribosomal RNA gene sites in diploid and polyploid
BMC Plant Biology, 2011 Background The genus Fragaria comprises species at ploidy levels ranging from diploid (2n = 2x = 14) to decaploid (2n = 10x = 70). Fluorescence in situ hybridization with 5S and 25S rDNA probes was performed to gather cytogenetic information that ...
Liu Bo, Davis Thomas M
doaj +1 more source