Results 301 to 310 of about 144,727 (384)

Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is a widely used delivery vehicle in gene therapy. A scalable production technology is essential for its wide clinical applications. We have taken a synthetic biology approach to generate HEK293‐based cell lines which harbor integrated genetic elements encoding essential AAV and adenoviral helper ...
Han‐Jung Kuo, Prahalad Srinivasan, Yu‐Chieh Lin, Min Lu, Carissa Rungkittikhun, Qi Zhang, Wei‐Shou Hu   +6 more
wiley   +1 more source

Circulating miRNAs correlate with clinical evaluation of activity in ANCA-associated glomerulonephritis. [PDF]

Eur J Med Res
Bošnjak M, Večerić-Haler Ž, Pipan Tkalec Ž, Boštjančič E, Kojc N.   +4 more
europepmc   +1 more source

Accelerating AAV purification process development using high‐throughput resin tip module

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated viruses (AAVs) with precise genome editing and cell‐virus interaction have become a promising delivery tool for gene therapy. A robust AAV purification process is crucial for ensuring therapeutic efficacy. The challenges of AAV purification process development encompass limited material availability during early ...
Qingxuan Li, Mahsa Hadidi, Steven Benner, Junfen Ma   +3 more
wiley   +1 more source

Development of cell-based assay for detecting replication-competent adeno-associated virus by qPCR. [PDF]

Mol Ther Methods Clin Dev
Shevade A, Reeves JS, Bak H, Tustian AD.
europepmc   +1 more source

Capsid Modifications for Targeting and Improving the Efficacy of AAV Vectors

Molecular Therapy: Methods & Clinical Development, 2019
H. Büning, A. Srivastava
semanticscholar   +1 more source

Harvesting AAV by tangential flow filtration using reverse asymmetric membranes

Biotechnology Progress, EarlyView.
Abstract Efficient bioreactor clarification for harvesting virus particles is often challenging. Tangential flow filtration is attractive as it can be easily adapted for batch and perfusion operations. Here the feasibility of using reverse asymmetric hollow fiber membranes, where the more open support structure faces the feed stream, has been ...
Xiaolei Hao, Ronny Horax, Xianghong Qian, April Wheeler, Hironobu Shirataki, S. Ranil Wickramasinghe   +5 more
wiley   +1 more source

Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti‐PD‐1 immunotherapy efficacy in colorectal cancer liver metastasis

Cancer Communications, EarlyView.
Abstract Background Colorectal cancer liver metastasis (CRLM) is characterized by an immunosuppressive microenvironment and a blunted response to immunotherapy. Notably, tumor‐associated macrophages (TAMs) play a critical role in modulating immune responses and exhibit significant heterogeneity in CRLM.
Yizhi Zhan, Jinsong Xu, Zhanqiao Zhang, Yating Hu, Yongsheng Li, Junying Qian, Yunyan Ling, Dehua Wu, Haijun Deng, Guoxin Li, Zhiyong Shen, Yuan Fang   +11 more
wiley   +1 more source

AAV6 vectors provide superior gene transfer compared to AAV9 vectors following intramyocardial administration. [PDF]

Mol Ther Methods Clin Dev
Wang J, Jonker T, Cervera-Barea A, Dong Z, Visser RN, Birza EE, Boender AR, Klerk M, Yang Y, Visser J, Jansen MS, Grootswagers TC, Bart CI, de Jager SCA, Schrödel S, Thirion C, Kirzner OF, Tan HL, de Vries AAF, Sluijter JPG, Neef K, de Groot JR, Christoffels VM, Boink GJJ.   +23 more
europepmc   +1 more source

Transcription factor EB activation rescues advanced αB-crystallin mutation-induced cardiomyopathy by normalizing desmin localization [PDF]

, 2019
Benjamin, Ivor J, Diwan, Abhinav, Foroughi, Layla, French, Brent A, Hill, Joseph A, Javeheri, Ali, Kovacs, Attila, Kraja, Aldi, Liu, Haiyan, Ma, Xiucui, Mani, Kartik, Murphy, John T, Weinheimer, Carla J   +12 more
core   +1 more source

Development and Recent Advances in SLIPT‐PM: A Chemogenetic Platform for Manipulating Signaling at the Plasma Membrane

ChemBioChem, EarlyView.
SLIPT‐PM enables the translocation of tag‐fused proteins from the cytoplasm to the plasma membrane using a synthetic self‐localizing ligand. This user‐friendly system permits reversible control of diverse signaling pathways. This concept article highlights the key features and current applications of SLIPT‐PM as a powerful tool in chemical and ...
Shuya Ohira, Akinobu Nakamura, Kenta Terai, Shinya Tsukiji   +3 more
wiley   +1 more source