Results 151 to 160 of about 204,301 (258)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Consistent diversity loss but divergent functional responses of freshwater biofilms to salinity and nutrient enrichment across climatic regions. [PDF]

Proc Biol Sci
Fuggle R, Mayer-Pinto M, Matias MG, Marzinelli EM.   +3 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Straw Returning Strategies and Decomposing Inoculant Addition Shape Microbial Communities and Functional Potential in Black Soil. [PDF]

Microorganisms
Wang C, Song T, Fu Z, Yang T, Wang W, Zhang T, Teng H.   +6 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Long-term salinity is associated with deteriorated soil properties, rhizosphere microbiome restructuring, and elevated wheat crown rot: evidence from a 19-year gradient experiment and a 3-year remediation verification. [PDF]

Front Plant Sci
Wang Y, Han S, Liu X, Dang H, Zheng C, Zheng Y, Cao C, Wu Y.   +7 more
europepmc   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Personalized whole-body modeling links gut microbiota to metabolic perturbations in Alzheimer's disease. [PDF]

Gut Microbes
Hensen T, Khatib L, Patel L, McDonald D, González A, MahmoudianDehkordi S, Blach C, Knight R, Kaddurah-Daouk R, Thiele I.   +9 more
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Estimating protein isoform abundances with [Formula: see text]. [PDF]

Proc Natl Acad Sci U S A
Testa L, Klei L, Rengle A, K Yocum A, Lewis DA, Devlin B, Roeder K, MacDonald ML.   +7 more
europepmc   +1 more source