Results 131 to 140 of about 3,715 (174)
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Carbohydrate analysis of Acanthamoeba castellanii
Experimental Parasitology, 2009We analyzed biochemically Acanthamoeba castellanii trophozoites, intact cysts and cyst walls belonging to the T4 genotype using gas chromatography combined with mass spectrometry. Cyst walls were prepared by removing intracellular material from cysts by pre-treating them with sodium dodecyl sulphate (SDS) containing dithiothreitol, and then subjecting ...
Ricky, Dudley +2 more
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Purine salvage by Acanthamoeba castellanii
International Journal for Parasitology, 1987Abstract Trophozoites of Acanthamoeba castellanii were found to incorporate a range of purine bases and nucleosides into parasite nucleic acids. Results from competition studies suggest that A. castellanii is capable of interconverting purine nucleotides.
H F, Hassan, G H, Coombs
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DIFFERENTIATION IN ACANTHAMOEBA CASTELLANII
Annual Review of Microbiology, 1976INTRODUCTION ........ ... 190 METHODS OF CULTURE AND ENCYSTMENT 193 A DESCRIPTION OF ENCYSTMENT IN EM 194 CONSIDERATION OF THE ROLE OF NUCLEIC ACIDS 200 Trophozoite DNA . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .....
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Acanthamoeba castellanii: Characterization of an Adhesin Molecule
Experimental Parasitology, 1999Acanthamoeba castellanii is a free-living protozoan that causes keratitis in humans and has been associated with pneumonia and granulomatous amebic encephalitis in dogs, sheep, and other species. Adherence of the Acanthamoeba to epithelial cells is critical to the pathogenesis of this disease.
M J, Kennett +3 more
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Glucolipid Synthesis in Acanthamoeba castellanii*
The Journal of Protozoology, 1976SYNOPSIS. Cell‐free preparations of Acanthamoeba castellanii trophozoites transfer glucose from UDP‐[U‐14C]glucose to a chloroform‐soluble form. This radioactive material has been isolated by thin‐layer chromatography; it contains an alkali‐labile and an alkali‐stable (unsaponifiable) component.
H B, Skrdlant, R A, Weisman
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Isolation of Giant Viruses of Acanthamoeba castellanii
Current Protocols, 2022AbstractThis article describes a practical method for prospecting and isolating giant viruses based on direct inoculation of environmental samples into amoeba cultures of Acanthamoeba castellanii. The giant viruses that infect amoebas have already been isolated from various environmental samples in several countries worldwide, including in extreme ...
Talita Bastos, Machado +2 more
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Encystment in Acanthamoeba castellanii: A review
Experimental Parasitology, 2014Differentiation of Acanthamoeba castellanii trophozoites involves massive turnover of cellular components and remodelling of organelle structure and function so as to produce a cryptobiotic cell, resistant to desiccation, heat, freezing, and chemical treatments.
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Protease activities of Acanthamoeba polyphaga and Acanthamoeba castellanii
Canadian Journal of Microbiology, 2006Acanthamoeba spp. are free-living amoebae that cause amoebic granulomatous encephalitis, skin lesions, and ocular amoebic keratitis in humans. Several authors have suggested that proteases could play a role in the pathogenesis of these diseases. In the present work, we performed a partial biochemical characterization of proteases in crude extracts of ...
José de Jesús, Serrano-Luna +5 more
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Acanthamoeba castellanii: Specificity of immobilization test
Experimental Parasitology, 1969Recognizable changes in the structure of Acanthamoeba castellanii (Neff strain)1 during antiserum immobilization are described and documented with phase-contrast photomicrographs. Antisera against A. castellanii used in this test failed to react with cultures of A. polyphaga, A. astronyxis, and A. palestinensis.
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Comparison of pinocytosis and phagocytosis in Acanthamoeba castellanii
Experimental Cell Research, 1977Abstract Acanthamoeba, with high rates of phagocytosis and pinocytosis of the non-concentrative type, offers favorable experimental material for investigation of similarities and possible differences in these two modes of uptake. Phagocytosis was measured by the rate of uptake of latex beads and pinocytosis by the rate of uptake of radioactive inulin
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