Evaluation of the epidemiological importance of classical swine fever infected, E2 sub-unit marker vaccinated animals with RT-nPCR positive blood samples [PDF]
<p>It has been demonstrated that pigs that have been double vaccinated with an E2 sub-unit marker vaccine and that are infected with classical swine fever virus (CSFV) through a natural contact infection may react positive in a CSFV detecting RT ...
Dewulf, Jeroen+5 more
core +2 more sources
Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson+12 more
wiley +1 more source
Micropropagation of a recalcitrant pine (Pinus pinea L.): An overview of the effects of ectomycorrhizal inoculation [PDF]
Stone pine (Pinus pinea L.) is an economically important forest species in some regions of Iberian Peninsula. Portugal and Spain have nearly 500,000 ha of stone pine stands, representing 85% of worldwide distribution.
Caldeira, A.T.+8 more
core
CD105‐LNPs and PS‐LNPs can deliver α‐lipoic acid to bone marrow mesenchymal stem cells and bone marrow‐derived macrophages, reversing high glucose‐induced oxidative stress while enhancing osteogenesis and M2 polarization. Integrating both nanoparticles into glucose‐responsive hyaluronic acid hydrogel forms DLNPs@HA hydrogel, which accelerates diabetic ...
Yanzhi Zhao+11 more
wiley +1 more source
Deinococcus radiodurans‐derived extracellular vesicles (R1‐EVs) provide radioprotection against total‐body irradiation‐induced acute radiation syndrome in mice. R1‐EVs mitigate oxidative damage by scavenging free radicals, promoting intestinal repair, enhancing hematopoietic function, and modulating immune responses. This study highlights the potential
Jeong Moo Han+7 more
wiley +1 more source
Commercial mariculture of Oreochromis niloticus using net cages [PDF]
The culture of tilapia has a long history in Africa. Fossil remains of members of the genus have been found which are about 18 million years old (Fryer and Iles, 1972).
Fineman-Kalio, A.S.
core
Do pingers cause stress in fish? An experimental tank study with European sardine, Sardina pilchardus (Walbaum, 1792) (Actinopterygii, Clupeidae), exposed to a 70 kHz dolphin pinger [PDF]
Date of acceptance: 06/12/2014 Acknowledgments The study was funded by the Portuguese Ministry of Science (Fundac¸a˜o para a Cieˆncia e Tecnologia– FCT) through a PhD Grant of SG (SFRH/BD/47931/2008).
Begona Santos, M.+5 more
core +1 more source
Lipid Nanoparticle‐Mediated CRISPR‐Cas13a Delivery for the Control of Bacterial Infection
New formulations of lipid nanoparticles (LNPs) that can deliver nucleic acids to Gram‐negative bacteria are proposed to combat bacterial infection. The delivery of nucleic acids by LNPs is aided by LNP‐helpers which weaken the bacterial outer membrane. LNPs encapsulating the Cas13a/gRNA expression vector achieve an antibacterial effect in both in vivo ...
Bookun Kim+9 more
wiley +1 more source
Islatravir‐loaded dissolving microarray patches (MAPs) provide a minimally invasive, long‐acting solution for HIV‐1 prevention and treatment. In rats, sustained islatravir delivery lasts up to three months, while in minipigs, efficacious plasma levels are detected for six days.
Qonita Kurnia Anjani+11 more
wiley +1 more source
Advancing our Understanding of Heat Wave Criteria and Associated Health Impacts to Improve Heat Wave Alerts in Developing Country Settings. [PDF]
Health effects of heat waves with high baseline temperatures in areas such as India remain a critical research gap. In these regions, extreme temperatures may affect the underlying population's adaptive capacity; heat wave alerts should be optimized to ...
Azhar, Gulrez Shah+6 more
core +2 more sources