Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Closing the motor vehicle industry - the impact on Australia [PDF]
The Australian Workplace Innovation and Social Research Centre (WISeR) commissioned a study by the National Institute of Economic and Industry Research (NIEIR) to assess the impact of the closure of the motor vehicle industry in Australia following ...
Bianca Barbaro +2 more
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An overview of peer-to-peer accommodation operation during the COVID-19 pandemic : responses, strategies and opportunities [PDF]
The outbreak of the COVID-19 pandemic had catastrophic impacts on many hospitality industries including the peer-to-peer (P2P) accommodation industry. The fast spread of the COVID-19 outbreak in countries throughout the world left very limited time for ...
Cui, Jianqiang, Attard, Maria
core +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
A Framework for Industry Export Competitiveness: Evidence from the Alcoholic Beverages Industry
Participation in international trade is a key indicator of an industry’s competitiveness. This study presents a framework for measuring the export competitiveness of an industry across countries that takes into account (a) industry specialization ...
Johnson, James P. +2 more
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Strategic Adaptation to Deregulation in the Indian Auto Components Industry [PDF]
We explore the strategic adaptation by domestic firms subsequent to economic liberalization and industry deregulation in an emerging economy. We study the Indian auto components industry during a ten-year period (1992-2002), using the Prowess database ...
Tripathy, Arindam +3 more
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Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Innovation Challenges in the Lighting Industry From 1990 to 2006 [PDF]
The lighting industry faces major competitive challenges with the introduction of new solid-state lighting technologies. Solid-state lighting’s greater energy efficiency, lifetime, and other features have led to widespread adoption in backlights ...
Sanderson, Susan +3 more
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