Results 11 to 20 of about 6,598 (201)

Metabolic Engineering of Escherichia coli for High-Level Production of (R)-Acetoin from Low-Cost Raw Materials

Microorganisms, 2023
Acetoin is an important four-carbon platform chemical with versatile applications. Optically pure (R)-acetoin is more valuable than the racemate as it can be applied in the asymmetric synthesis of optically active α-hydroxy ketone derivatives ...
Mengxue Diao, Xianrui Chen, Jing Li, Ya’nan Shi, Bo Yu, Zhilin Ma, Jianxiu Li, Nengzhong Xie   +7 more
doaj   +3 more sources

Mechanisms of Acetoin Toxicity and Adaptive Responses in an Acetoin-Producing Species, Lactococcus lactis

Applied and Environmental Microbiology, 2021
Acetoin, 3-hydroxyl,2-butanone, has diverse uses in chemical industry, agriculture, and dairy industries as a volatile compound that generates aromas. In bacteria, it can be produced in high amount by Lactococcus lactis when it grows under aerobic respiration.
Cesselin, Bénédicte, Henry, Céline, Gruss, Alexandra, Gloux, Karine, Gaudu, Philippe   +4 more
semanticscholar   +6 more sources

Metabolic Engineering of Zymomonas mobilis for Acetoin Production by Carbon Redistribution and Cofactor Balance

Fermentation, 2023
Biorefinery to produce value-added biochemicals offers a promising alternative to meet our sustainable energy and environmental goals. Acetoin is widely used in the food and cosmetic industries as taste and fragrance enhancer.
Weiwei Bao, Wei Shen, Qiqun Peng, Jun Du, Shihui Yang   +4 more
doaj   +2 more sources

Two-stage pH control strategy based on the pH preference of acetoin reductase regulates acetoin and 2,3-butanediol distribution in Bacillus subtilis. [PDF]

PLoS ONE, 2014
Acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), which catalyzes the interconversion between acetoin and 2,3-butanediol, plays an important role in distribution of the products pools.
Xian Zhang, Teng Bao, Zhiming Rao, Taowei Yang, Zhenghong Xu, Shangtian Yang, Huazhong Li   +6 more
doaj   +2 more sources

Metabolic engineering for the production of acetoin and 2,3-butanediol at elevated temperature in Parageobacillus thermoglucosidasius NCIMB 11955

Frontiers in Bioengineering and Biotechnology, 2023
The current climate crisis has emphasised the need to achieve global net-zero by 2050, with countries being urged to set considerable emission reduction targets by 2030.
Lili Sheng, Abubakar Madika, Abubakar Madika, Matthew S. H. Lau, Ying Zhang, Nigel P. Minton, Nigel P. Minton   +6 more
doaj   +2 more sources

Characterization and Regulation of the Acetolactate Synthase Genes Involved in Acetoin Biosynthesis in Acetobacter pasteurianus

Foods, 2021
Acetoin is an important aroma-active chemical in cereal vinegars. Acetobacter pasteurianus was reported to make a significant contribution to acetoin generation in cereal vinegars.
Jingyi Zhao, Zhe Meng, Xiaolong Ma, Shumei Zhao, Yang An, Zijun Xiao   +5 more
doaj   +2 more sources

Effect of alsD deletion and overexpression of nox and alsS on diacetyl and acetoin production by Lacticaseibacillus casei during milk fermentation

Journal of Dairy Science, 2022
: Diacetyl and acetoin are key aroma components of fermented milk but are produced in low concentrations by starter cultures. In this study, we expressed NADH oxidase, acetolactate synthase, and inactivated acetolactate decarboxylase in ...
Huaixiang Tian, Yan Jing, Haiyan Yu, Juan Huang, Haibin Yuan, Xinman Lou, Bei Wang, Zhiyuan Xu, Chen Chen   +8 more
doaj   +2 more sources

Analysis of heterologous expression of phaCBA promotes the acetoin stress response mechanism in Bacillus subtilis using transcriptomics and metabolomics approaches

Microbial Cell Factories
Acetoin, a versatile platform chemical and popular food additive, poses a challenge to the biosafety strain Bacillus subtilis when produced in high concentrations due to its intrinsic toxicity.
Tao Li, Haixiang Li, Lei Zhong, Yufei Qin, Gege Guo, Zhaoxing Liu, Ning Hao, Pingkai Ouyang   +7 more
doaj   +2 more sources

Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae

Biotechnology for Biofuels, 2021
Background Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasingly important as a sustainable ...
Hsiang-Yen Su, Hua-Ying Li, Cai-Yun Xie, Qiang Fei, Ke-Ke Cheng   +4 more
doaj   +2 more sources

Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum

Microbial Cell Factories, 2020
Background Acetoin, especially the optically pure (3S)- or (3R)-enantiomer, is a high-value-added bio-based platform chemical and important potential pharmaceutical intermediate.
Lingxue Lu, Yufeng Mao, Mengyun Kou, Zhenzhen Cui, Biao Jin, Zhishuai Chang, Zhiwen Wang, Hongwu Ma, Tao Chen   +8 more
doaj   +2 more sources