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The hydrolysis of bile acid conjugates

Steroids, 1978
Studies were made of a) the relationship of bile acid structure and analytical recoveries (measured by 3-hydroxysteroid oxidoreductase) following vigorous alkaline hydrolysis of bile acid conjugates and b) the relationship of structure and hydrolysis time of taurine- and glycine bile acid conjugates in a reaction catalyzed by glycocholic acid hydrolase.
W T, Beher   +3 more
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Feulgen Hydrolysis with Phosphoric Acid

Stain Technology, 1953
Sections from rat tissues fixed in a 10% solution of formalin in 90% alcohol were treated with phosphoric acid in concentrations varying from 30 to 85% at room temperature (28°), and subsequently stained with the Schiff reagent. Intense staining of the nuclear material was obtained in 5 to 15 minutes when 40 to 75% phosphoric acid was used. The intense
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The Hydrolysis Rate of Sulphamic Acid

Transactions of the IMF, 1973
AbstractThe rate of hydrolysis of sulphamic acid has been measured over the pH and temperature range of interest to electroplaters. The results have been correlated with accurate results at other temperatures reported in the literature, and are presented as a plot of half‐life of sulphamic acid as a function of pH, from 50 to 95 °C, and as a plot of ...
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Hydrolysis of phospholipids with hydrochloric acid

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
Abstract The hydrolysis of dl -α-lecithin, dl -α-cephalin and sphingomyelin with 2 N HCl was investigated. The main hydrolytic products were identified and quantitatively estimated. From the data obtained a reaction mechanism for the acid hydrolysis of the above-mentioned compounds is postulated.
A J, De Koning, K B, McMullan
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Acid hydrolysis of monoclonal antibodies

Journal of Immunological Methods, 1995
For analysis of monoclonal antibodies using polyacrylamide gel electrophoresis, two hydrolytic fragments derived from the heavy chain of mouse IgG1 were produced during incubation of the antibodies in Laemmli reducing sample buffer at 100 degrees C for 5 min. The cleavage sites were identified by amino terminal sequencing.
J, Davagnino   +3 more
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Hydrolysis of Chitosan in Lactic Acid

Applied Biochemistry and Microbiology, 2004
Effects of molecular weight and degree of acetylation on the hydrolysis of chitosan in dilute lactic acid were studied. It was demonstrated that the higher were the values of both parameters, the more rapid were the decreases in viscosity and viscosity-average molecular weight of chitosan.
A V, Il'ina, V P, Varlamov
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Acidic hydrolysis of pyrimidine deoxyribonucleotides

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Abstract The rates of acidic N-glycosyl cleavage of the 3′- and 5′-phosphates of deoxycytidine and thymidine have been studied. The pH-rate profiles and thermodynamic parameters for the hydrolyses resemble those of the parent nucleosides. It is likely that the A-1 mechanism observed with the nucleosides also operates at the nucleotide level.
R, Shapiro, M, Danzig
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Enzymatic hydrolysis of alginic acid

Biochimica et Biophysica Acta, 1954
Abstract An alginic acid hydrolizing bacterium has been isolated which excretes an enzyme capable of degrading alginic acid into mannuronic acid. A series of intermediary oligomannuronides has been demonstrated by paper chromatography to be present during enzymatic ...
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Kinetics of enzymic hydrolysis of malto-oligosaccharides: A comparison with acid hydrolysis

Carbohydrate Research, 1987
Abstract The hydrolysis of malto-oligosaccharides G 3 -G 6 catalysed by porcine pancreatic alpha-amylase was investigated kinetically at 25°. Kinetic parameters corresponding to different positions of enzymic attack were determined and product inhibition was evaluated.
Beltrame, P.L.   +6 more
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Mechanisms of the Feulgen acid hydrolysis

Journal of Microscopy, 1980
SUMMARYOur present knowledge of the two main reactions responsible for the form of the Feulgen hydrolysis curve is summarized. A suggestion that hydrolysis slightly above room temperature and in 5 M HCl is preferable is made and discussed. A formula making it possible to calculate the appropriate hydrolysis time at any normally used acid concentration ...
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