Results 251 to 260 of about 3,054,380 (300)

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

Advanced Science, EarlyView.
The promoted Escherichia coli‐assisted continuous evolution (PEACE) system operates through a stringent three step genetic circuit: target gene mutation via a deaminase–T7 RNA polymerase (T7 RNAP) fusion, coupling variant function to antitoxin expression, and growth based selection of surviving cells.
Xinyu Zhang, Zhanzhi Liu, Ying Xu, Mingjing Sun, Guangzhen Zhou, Deming Rao, Xiaoqian Chen, Sheng Wang, Zhigang Li, Yan Huang, Jing Wu   +10 more
wiley   +1 more source

Rapid Multiplex Antimicrobial Resistance Profiling and Bacterial Identification by LAP‐MALDI Mass Spectrometry Biotyping

Advanced Science, EarlyView.
Liquid atmospheric pressure matrix‐assisted laser desorption/ionization (LAP‐MALDI) mass spectrometry (MS) provides multiplexed biotyping of microbial cultures using a fast and simple workflow. This is demonstrated for clinically relevant bacteria, resulting in accurate antimicrobial resistance (AMR) detection, speciation by MS/MS sequencing and ...
Lily R. Adair, Shabnam Iyer, Ian M. Jones, Rainer Cramer   +3 more
wiley   +1 more source

Drop‐Location‐Insensitive Droplet‐Based Electricity Generator Using Polydimethylsiloxane Slippery Covalently Attached Liquid Coatings

Advanced Science, EarlyView.
Drop‐location sensitivity remains a key barrier to practical droplet‐based electricity generators (DEGs). Here, a polydimethylsiloxane slippery covalently attached liquid (PDMS‐SCAL)‐coated interface promotes droplet sliding instead of splitting or bouncing, allowing stable electricity generation from randomly falling raindrops. The resulting PDMS‐SCAL‐
Jung Bin Yang, Donghyeon Yoo, Hideaki Teshima, Donghan Lee, Dongwhi Choi, Dong Rip Kim, Nenad Miljkovic   +6 more
wiley   +1 more source

Lanthanum Carbonate Nanosheets Based Colloidal Hydrogel for Modulating Innate & Adaptive Tumor Immunotherapy by Augmented Cellular Pyroptosis

Advanced Science, EarlyView.
The Gela/decitabine/La2(CO3)3 colloidal (GDLC) hydrogel developed here could reverse the epigenetic silencing of the GSDME gene by the synergistic interaction between DAC and La3+, while concurrently activating caspase‐3, thus facilitating pyroptosis induction under the tumor microenvironment.
Yang Hong, Haitao Wu, Weitao Wang, Xuan Zhang, Qian Chen, Yaoyu Hu, Zhengbao Zha   +6 more
wiley   +1 more source

Polymer Concepts in Cellular Function

Advanced Science, EarlyView.
Miao Yu, Shikha Dhiman, Dorothee Dormann, Frauke Gräter, Katja Luck, Friederike Schmid, Andreas Walther, Edward A. Lemke   +7 more
wiley   +1 more source

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The acid hydrolysis of glycosides

Carbohydrate Research, 1967
Abstract Isopropyl and 2-chloroethyl 3-O-methyl-β- d -glucopyranosiduronic acids, but not the corresponding β- d -glucopyranosides, are hydrolyzed in acid solution at a rate higher than that for the unmethylated compounds. The hydrolysis of 6-O-methyl-β- d -glucopyranosides is independent of the polarity of the aglycon. Several 5-C-substituted methyl
M.D. Saunders, T.E. Timell
openaire   +1 more source

Acid Hydrolysis of Sulphapyridines

Nature, 1946
SULPHAPYRIDINE (M and B 693) is readily hydrolysed by dilute mineral acids1. Many isomerides of sulphapyridine have been made and it has been found that the three isomeric derivatives from 3-aminopyridine (3-(o-, m- and p-aminobenzenesulphonamido)pyri-dine) are stable to hot mineral acids.
openaire   +2 more sources

Acid hydrolysis of monoclonal antibodies

Journal of Immunological Methods, 1995
For analysis of monoclonal antibodies using polyacrylamide gel electrophoresis, two hydrolytic fragments derived from the heavy chain of mouse IgG1 were produced during incubation of the antibodies in Laemmli reducing sample buffer at 100 degrees C for 5 min. The cleavage sites were identified by amino terminal sequencing.
J, Davagnino, C, Wong, L, Shelton, S, Mankarious   +3 more
openaire   +2 more sources

Hydrolysis of phospholipids with hydrochloric acid

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
Abstract The hydrolysis of dl -α-lecithin, dl -α-cephalin and sphingomyelin with 2 N HCl was investigated. The main hydrolytic products were identified and quantitatively estimated. From the data obtained a reaction mechanism for the acid hydrolysis of the above-mentioned compounds is postulated.
A J, De Koning, K B, McMullan
openaire   +2 more sources

Feulgen Hydrolysis with Phosphoric Acid

Stain Technology, 1953
Sections from rat tissues fixed in a 10% solution of formalin in 90% alcohol were treated with phosphoric acid in concentrations varying from 30 to 85% at room temperature (28°), and subsequently stained with the Schiff reagent. Intense staining of the nuclear material was obtained in 5 to 15 minutes when 40 to 75% phosphoric acid was used. The intense
openaire   +2 more sources