Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Simulations of a Current-Source Shunt Active Power Filter with Carrier-Based PWM and Periodic Sampling modulation techniques [PDF]
Bruno Exposto, Helder Carneiro, J. G. Pinto, Carlos Couto, João L. Afonso, "Simulations of a current-source Shunt Active Power Filter with Carrier-Based PWM and Periodic Sampling Modulation Techniques" Proceedings of the 2011-14th European Conference on ...
Afonso, João L. +4 more
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A kind of digital single-cycle control method of active power filter
The paper analyzed working principle of active power filter, derived mathematical model of single-cycle control of three-phase three-wire active power filter. It combined digital single-cycle control and ip-iq harmonic current detection method to realize
ZHENG Feng, LI La
doaj +1 more source
Shunt active filter for power quality improvement [PDF]
This paper describes the development of a low cost shunt active power filter with digital control, which allows dynamic power factor correction and both harmonics and zero-sequence current compensation.
Afonso, João L. +3 more
core
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
A PV powered shunt active power filter for power quality improvement
This paper deals with power quality improvement using a three-phase active power filter (APF) connected to a PV power system. A direct power control (DPC) approach is proposed to eliminate harmonic current caused by any nonlinear loads and at the same ...
Ayoub Benzahia +5 more
doaj +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Experimental and simulation results of a current-source three-phase shunt active power filter using periodic-sampling [PDF]
In this paper are presented experimental and simulation results of a Current-Source Three-Phase Shunt Active Power Filter, during its operation to compensate the currents a low power factor load and a nonlinear load.
Afonso, João L. +4 more
core

