Results 51 to 60 of about 5,966,150 (254)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Using the MAPS-Global Audit Tool to Assess the Influence of Microscale Built-Environment Attributes Related to Physical Activity and Sedentary Behavior in Spanish Youth

Safety
Environmental factors have been identified as having a direct relationship with physical activity (PA) and sedentary behavior. The main aim of this study was to investigate the relationship between microscale built-environment attributes and the levels ...
Marta Terrón-Pérez, Javier Molina-García, Elena Santainés-Borredá, Isaac Estevan, Ana Queralt   +4 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Adolescent physical activity during school days: a comparative study before and after COVID-19 pandemic restrictions

Frontiers in Public Health
BackgroundPhysical activity (PA) during the school day is crucial for the health and well-being of adolescents. This study examines the impact of the COVID-19 pandemic on youth PA patterns to better understand these changes and to provide guidelines for ...
Karel Frömel, Karel Frömel, Gregory Welk, Dorota Groffik, Lukáš Jakubec, Jan Dygrýn, Josef Mitáš   +6 more
doaj   +1 more source

Dendritic trafficking faces physiologically critical speed-precision tradeoffs

eLife, 2016
Nervous system function requires intracellular transport of channels, receptors, mRNAs, and other cargo throughout complex neuronal morphologies. Local signals such as synaptic input can regulate cargo trafficking, motivating the leading conceptual model
Alex H Williams, Cian O'Donnell, Terrence J Sejnowski, Timothy O'Leary   +3 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Active Commuting to University and its Association with Sociodemographic Factors and Physical Activity Levels in Chilean Students

Medicina, 2019
Background and Objectives: Active commuting to and from university (ACU) could be a strategy to increase physical activity levels (PA) and promote health in young university students.
Yaira Barranco-Ruiz, Carolina Cruz León, Emilio Villa-González, Ximena Palma Leal, Palma Chillón, Fernando Rodríguez-Rodríguez   +5 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Supporting Mobility Across European Cities through Physically Active-Friendly Urban Environments

ARCHive-SR, 2018
Active transport i.e. cycling or walking as well as using public transport for everyday journeys is an effective approach with multiple social and environmental benefits for transforming urban environments into active urban environments. Although cycling
Rosina U. Ndukwe
doaj   +1 more source