Results 71 to 80 of about 30,326 (264)

On OCT Image Classification via Deep Learning

IEEE Photonics Journal, 2019
Computer-aided diagnosis of retinopathy is a research hotspot in the field of medical image classification. Diabetic macular edema (DME) and age-related macular degeneration (AMD) are two common ocular diseases that can result in partial or complete loss
Depeng Wang, Liejun Wang
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Ocular Clocks: Adapting Mechanisms for Eye Functions and Health

Investigative Opthalmology & Visual Science, 2018
Vision is a highly rhythmic function adapted to the extensive changes in light intensity occurring over the 24-hour day. This adaptation relies on rhythms in cellular and molecular processes, which are orchestrated by a network of circadian clocks located within the retina and in the eye, synchronized to the day/night cycle and which, together, fine ...
Felder-Schmittbuhl, Marie-Paule, Buhr, Ethan, Dkhissi-Benyahya, Ouria, Hicks, David, Peirson, Stuart, Ribelayga, Christophe, Sandu, Cristina, Spessert, Rainer, Tosini, Gianluca   +8 more
openaire   +4 more sources

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

High-resolution genomics uncovers region-specific evolution and virulence of ocular Streptococcus pneumoniae

Microbiology Spectrum
Streptococcus pneumoniae is the leading cause of sight-threatening ocular infection. However, comprehensive molecular epidemiological analyses that focus on ocular pneumococci are limited.
Xi Xiang, Mengke Ye, Shouyuan Chen, Lin Liu, Tingting Hong, Dongping Hu, Lihong Ge, Ying Fu, Feng Zhao, Yanfei Wang, Lihong Bo, Jun Lu, Yan Jiang, Yunsong Yu, Xueqing Wu   +14 more
doaj   +1 more source

Impairment of Long-Term Plasticity of Cerebellar Purkinje Cells Eliminates the Effect of Anodal Direct Current Stimulation on Vestibulo-Ocular Reflex Habituation

Frontiers in Neuroscience, 2017
Anodal direct current stimulation (DCS) of the cerebellum facilitates adaptation tasks, but the mechanism underlying this effect is poorly understood. We have evaluated whether the effects of DCS effects depend on plasticity of cerebellar Purkinje cells (
Suman Das, Suman Das, Suman Das, Marcella Spoor, Tafadzwa M. Sibindi, Peter Holland, Peter Holland, Martijn Schonewille, Chris I. De Zeeuw, Chris I. De Zeeuw, Maarten A. Frens, Maarten A. Frens, Opher Donchin, Opher Donchin   +13 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Deployment of spatial attention without moving the eyes is boosted by oculomotor adaptation

Frontiers in Human Neuroscience, 2015
Vertebrates developed sophisticated solutions to select environmental visual information, being capable of moving attention without moving the eyes. A large body of behavioural and neuroimaging studies indicate a tight coupling between eye movements and ...
Ouazna eHabchi, Elodie eREY, Romain eMATHIEU, Christian eUrquizar, Alessandro eFARNE, Denis ePélisson   +5 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source