Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Support needs and adaptive behavior surveys: Services prediction and relationship. [PDF]
Piccorelli AV +4 more
europepmc +1 more source
Cognition, academic achievement, and adaptive behavior in school-aged girls with fragile X syndrome. [PDF]
Jordan TL +4 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Prefrontal cortex interneurons and their contributions to attention, working memory, and adaptive behavior. [PDF]
Boroujeni KB +3 more
europepmc +1 more source
Neurodevelopmental status and adaptive behavior of pediatric patients with mucopolysaccharidosis II: a longitudinal observational study. [PDF]
Muenzer J +9 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Adaptive behavior in older adults according to age and schooling. [PDF]
Tapia Albornoz C +6 more
europepmc +1 more source
Ketogenic diet for epilepsy control and enhancement in adaptive behavior. [PDF]
El-Rashidy OF +3 more
europepmc +1 more source

