Results 91 to 100 of about 60,047 (288)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Adaptive instruction in teaching and learning: evidence from junior high school teachers in the Jirapa Municipality of the Upper West Region of Ghana

Frontiers in Education
This study examined adaptive instruction in the classroom from teachers’ perspectives in the Jirapa Municipality of Ghana. A concurrent mixed-methods design was adopted.
Libanus Susan, Ebenezer Nabiebakye, Jane-Frances Yirdong Lobnibe   +2 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Accounting for physical barriers in insect pest modeling: A spatially explicit simulation-based approach

Ecological Informatics
Understanding pest dynamics beyond greenhouse boundaries is critical for anticipating outbreaks and guiding sustainable management. Despite the central role of insect pest ecology in Integrated Pest Management, landscape-scale research on external ...
Juan M. Requena-Mullor, Estefanía Rodríguez, Mónica González, Antonio J. Castro, Enrica Garau, Irene Pérez-Ramírez, Álvaro Peláez-Pérez, Pablo Barranco   +7 more
doaj   +1 more source

Energy-Efficient Data Collection Scheme for Environmental Quality Management in Buildings

IEEE Access, 2018
How to efficiently collect sensory data for supporting energy-efficient operation of buildings has become a great challenge, especially for large-scale networks in buildings.
Siguang Chen, Jiasheng Zhou, Xiaoyao Zheng, Xiukai Ruan   +3 more
doaj   +1 more source

Inverse Adaptive Cluster Sampling with Unequal Selection Probabilities: Case Studies on Crab Holes and Arsenic Pollution

, 2015
Adaptive cluster sampling is an efficient method of estimating the parameters of rare and clustered populations. The method mimics how biologists would like to collect data in the field by targeting survey effort to localised areas where the rare ...
Al Khayat, Jassim A., Jennifer Brown, Mohammad Moradi, Moradi, Mohammad, Brown, Jennifer, Mohammad Salehi, Yousif, Adil Eltayeb Mohamed, Jassim A. Al Khayat, Salehi, Mohammad, Adil Eltayeb Mohamed Yousif   +9 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Keypoint Detection Based on Curvature Grouping and Adaptive Sampling

IEEE Access
In the keypoint detection algorithm, the farthest point sampling methods and random sampling methods are usually used to select candidate points, then keypoints are screened out from the neighborhood of the candidate points.
Bifu Li, Yu Cheng, Weitong Li
doaj   +1 more source

CO2 emissions related to the Yellowstone volcanic system: 1. Developing a stratified adaptive cluster sampling plan

, 2000
Statistical sampling theory offers several sampling designs that are well-suited to geophysical research. Simple random sampling is the most commonly used formal methodology, in which the probability that any unit is sampled is known. Generally, units to
Boomer, K B, Brantley, S. L., Werner, Cindy, S. Brantley, C. Werner, K. Boomer   +5 more
core   +1 more source