Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Aerodynamic performance of a ventilation system for droplet control by coughing in a hospital isolation ward. [PDF]
Song Y +6 more
europepmc +1 more source
Circulation control propellers for general aviation, including a BASIC computer program [PDF]
The feasibility of replacing variable pitch propeller mechanisms with circulation control (Coanada effect) propellers on general aviation airplanes was examined.
Braslow, A. L. +2 more
core +1 more source
Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner +6 more
wiley +1 more source
Inhalable Nano-Dimpled Microspheres Containing Budesonide-PLGA for Improved Aerodynamic Performance. [PDF]
Han CS +4 more
europepmc +1 more source
Excess Ca2+ ions activate the Calcium‐Sensing Receptor (CaSR), which subsequently drives the uptake of excess inorganic phosphate (Pi) via the Pi transporter (Pit−1) in chondrocytes. This mechanism causes a toxic increase in intracellular Pi concentration, ultimately leading to chondrocyte apoptosis and pathological mineralization. Excess extracellular
Sachie Nakatani +7 more
wiley +1 more source
Dwarf Kingfisher-Inspired Bionic Flapping Wing and Its Aerodynamic Performance at Lowest Flight Speed. [PDF]
Abas MFB +5 more
europepmc +1 more source
Feasibility study for a numerical aerodynamic simulation facility. Volume 1 [PDF]
A Numerical Aerodynamic Simulation Facility (NASF) was designed for the simulation of fluid flow around three-dimensional bodies, both in wind tunnel environments and in free space.
Bergman, R. O. +9 more
core +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Research on Aerodynamic Performance of Bionic Fan Blades with Microstructured Surface. [PDF]
Gao M +6 more
europepmc +1 more source

