Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast [PDF]
A combined multidimensional chromatography-mass spectrometry approach known as "MudPIT" enables rapid identification of proteins that interact with a tagged bait while bypassing some of the problems associated with analysis of polypeptides excised from ...
Deshaies, Raymond J. +6 more
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Characterization of Cell Glycocalyx with Mass Spectrometry Methods. [PDF]
The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function.
Lebrilla, Carlito B +3 more
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Label‐Free Detection of a Neurotransmitter Using an Aptamer‐Functionalized Amorphous IGZO Transistor
Advanced Materials Interfaces, EarlyView.An aptamer‐functionalized amorphous IGZO thin‐film transistor enables label‐free electrical detection of the neurotransmitter serotonin under liquid‐gated operation. Stepwise surface functionalization ensures stable biomolecule integration and efficient electrostatic coupling.
Ngoc Thanh Ho +3 more
wiley +1 more source
An Advanced Molecularly Imprinted Polymer‐Based Electrochemical Aptasensor for Carbendazim Detection
Advanced Materials Interfaces, EarlyView.A dual‐recognition electrochemical aptasensor that integrates a molecular imprinted polymer (MIP) and an aptamer is developed for the detection of a common pesticide, carbendazim (CBZ). The resulting sensor exhibits enhanced selectivity toward CBZ over structurally similar compounds and achieves a low detection limit of 1.3 nm, demonstrating its ...
Suthira Pushparajah +3 more
wiley +1 more source
Bioengineering & Translational MedicineLentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for ...
Eduardo Barbieri +14 more
doaj +1 more source
A–C Estrogens as Potent and Selective Estrogen Receptor-Beta Agonists (SERBAs) to Enhance Memory Consolidation under Low-Estrogen Conditions [PDF]
Estrogen receptor-beta (ERβ) is a drug target for memory consolidation in postmenopausal women. Herein is reported a series of potent and selective ERβ agonists (SERBAs) with in vivo efficacy that are A–C estrogens, lacking the B and D estrogen rings ...
Donaldson, William A. +12 more
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Interface‐Engineered Binary Framework Composites: Advancing Porous Materials for Precision Medicine
Advanced Materials Interfaces, EarlyView.Binary framework composites integrate two complementary porous architectures into a unified platform, enabling multifunctional design, enhanced structural tunability, and improved physicochemical performance. By combining high surface area, ordered porosity, interfacial synergy, and versatile functionalization, these hybrid materials offer new ...
Navid Rabiee +3 more
wiley +1 more source
The development of a purification procedure for Peptide:n-Glycosidase A from Prunus amygdalus : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
Peptide-N4-acetyl-β-glucosaminyl) asparagine amidases cleave the amide bond between N-linked glycans at N-acetylglucosamine and asparagine, liberating intact oligosaccharide chains from glycoproteins. Although PNGase A is commonly used by glycobiologists
Diepen, Angela Corinne van
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Reconnaissance Peptide Labeling Grain Boundary of Chemically Grown MoS2 Polycrystalline Monolayer
Advanced Materials Technologies, EarlyView.Self‐assembled peptides on substrates, through adsorption and aggregation, offer an alternative way to label grain boundaries in chemically grown single‐layer polycrystalline MoS2. During an early nucleation step, peptides preferentially bind to grain boundaries.
Linhao Sun, Jinhua Hu
wiley +1 more source
Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research
Research in Molecular Medicine, 2016 Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin +8 more
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