Results 101 to 110 of about 533,930 (303)
Analyzing peptides and proteins by mass spectrometry: principles and applications in proteomics [PDF]
Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166The study of proteins has been a key element in biomedicine and biotechnology because of their important role in cell functions or enzymatic activity. Cells are the basic unit of living
Estanyol i Ullate, Josep Maria +3 more
core
Generation and in vivo characterization of a chimeric αvβ5-targeting antibody 14C5 and its derivatives [PDF]
Background: Previous studies showed that radiolabeled murine monoclonal antibody (mAb) 14C5 and its Fab and F(ab')2 fragments, targeting αvβ5 integrin, have promising properties for diagnostic and therapeutic applications in cancer.
De Vos, Filip +5 more
core +1 more source
Advanced Healthcare Materials, EarlyView.The image shows a schematic form a nanobody‐conjugated theranostic prodrug (NBD) platform targeting tumor‐associated αvβ3 integrin. The NBD system integrates selective nanobody‐mediated tumor recognition, glutathione‐responsive disulfide cleavage for doxorubicin release, and aza‐BODIPY‐based near‐infrared fluorescence for real‐time imaging.
Sanu Karan +13 more
wiley +1 more source
New AMD3100 derivatives for CXCR4 chemokine receptor targeted molecular imaging studies: synthesis, anti-HIV-1 evaluation and binding affinities [PDF]
CXCR4 is a target of growing interest for the development of new therapeutic drugs and imaging agents as its role in multiple disease states has been demonstrated.
Archibald, Stephen J. +14 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Sweetosomes, a new class of fucose‐decorated liposomes, are developed via a one‐step microfluidic process without surface chemistry. This study elucidates their main caveolae‐mediated entry and distinct endosomal trafficking. These nanostructures demonstrate superior endosomal escape, organelle acidity modulation, and prolonged plasma persistence ...
Mattia Tiboni +13 more
wiley +1 more source
MethodsXWe present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza +2 more
doaj +1 more source
Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines [PDF]
A powerful and potentially general approach to the targeting and crystallization of proteins on lipid interfaces through coordination of surface histidine residues to lipid-chelated divalent metal ions is presented.
Arnold, Frances H. +7 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Single‐chain nanoparticles have been proposed as drug delivery carriers, but adding a tail can significantly enhance their performance. This was demonstrated using a tadpole‐like structure comprising a head prepared from a UV‐crosslinked fructose‐based glycopolymer capable of targeting GLUT receptors, combined with a PEG‐based tail. Compared with other
Hoang Yen Vo +6 more
wiley +1 more source
Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target [PDF]
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Arcus, Vickery L. +3 more
core +1 more source
Advanced Materials, EarlyView.Ionic epoxy networks are prepared using ammonium salts as hardeners, leading to a two‐stage curing process with a thermoplastic‐like intermediate. This uncommon behavior enables extrusion and fabrication of thermoplastic prepregs that can be cured into thermoset composites.
Izabela Kurowska +10 more
wiley +1 more source