Results 101 to 110 of about 308,685 (162)

Lectin Affinity Chromatography

Molecular Biotechnology, 1994
Lectins are glycoproteins or proteins that have a selective affinity for a carbohydrate, or a group of carbohydrates. Many purified lectins are readily available and these maybe immobilized to a variety of chromatography supports.
I, West, O, Goldring
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Lectin Affinity Chromatography

2023
Glycosylation is a non-template-driven posttranslational modification during which linked-sugars and glycans are added to the nascent polypeptide. Over 70% of the eukaryotic proteome is thought to be glycosylated. It is now known that correct glycosylation is essential for the correct folding, solubility, stability, and immunogenicity of proteins.
Brendan F, O'Connor, Donal, Monaghan, Jonathan, Cawley   +2 more
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Receptor-affinity chromatography

Nature, 1988
Based upon the specificity and reversibility of receptor–ligand interactions, receptor-affinity chromatography is one of the newest affinity methods for the purification of biomolecules.
P, Bailon, D V, Weber
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The affinity chromatography of transketolase

Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
A number of possible affinity adsorbents for transketolase (sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphateglycolaldehydetransferase, EC 2.2.1.1) were prepared. The behaviour of the enzyme from Candida utilis and from Baker's yeast on columns of these and of Blue Sepharose CL-6B was examined, together with the behaviour of the contaminating ...
T, Wood, S, Fletcher
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Weak-affinity chromatography

Nature, 1990
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
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Receptor Affinity Chromatography

Annals of the New York Academy of Sciences, 1985
Insulin receptor was purified in high yield from cultured 3T3-L1 mouse adipocytes using the bifunctional ligand N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin in conjunction with avidin-Sepharose CL-4B. This ligand is 100% competent as insulin and 60% competent as biotin, as measured by competitive binding assays.
R A, Kohanski, M D, Lane
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Centrifugal affinity chromatography

Journal of Chromatography A, 1990
A new technique termed centrifugal affinity chromatography (CAC) is presented in this paper. CAC combines a high flow-rate, created by centrifugal force, with the specificity of affinity chromatography. This technique has been used for the purification of human immunoglobulin G.
R J, Slingerland, V, Adámek, W H, Scouten   +2 more
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Affinity chromatography of thrombin

Biochimica et Biophysica Acta (BBA) - Enzymology, 1971
Bovine thrombin (EC 3.4.4.13) has been purified by adsorption onto p-chlorobenzylamido-e-aminocaproyl agarose, the ligand having been covalently bound to agarose by the cyanogen bromide technique. Thrombin was eluted from the adsorbant with 1.0 M benzamidine and was recovered by gel filtration through Sephadex G-25 at high ionic strength to prevent ...
A R, Thompson, E W, Davie
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Affinity chromatography of glycosyltransferases

Journal of Chromatography A, 1981
This review summarizes the use of biospecific chromatography techniques in the purification of mammalian glycosyltransferases. Ligands that are analogues of donor or acceptor substrates have been linked to cyanogen bromide-activated agarose for use as affinity adsorbents.
J E, Sadler, T A, Beyer, R L, Hill
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Affinity chromatography of phosphofructokinase

Archives of Biochemistry and Biophysics, 1976
Abstract The behavior of mammalian phosphofructokinase on immobilized adenine nucleotides was investigated. Three different insolubilized ligands were compared using a pure rabbit muscle phosphofructokinase. N 6 -[(6-aminohexyl)-carbamoyl-methyl]-ATP-Sepharose bound at least 90 times more enzyme than either N 6 -(6-aminohexyl)-AMP-agarose or ATP ...
C S, Ramadoss, L J, Luby, K, Uyeda
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