Results 81 to 90 of about 1,195,639 (276)

Morphology, Transport, and Dynamics of Protein Adsorption in Open‐Cell Metal Foam

Advanced Engineering Materials, EarlyView.
Stainless steel (SS) open‐cell foams are shown to adsorb more protein per unit area than previously reported 316L SS and chromium oxide surfaces under static and flow conditions. An integrated approach combining 3D pore imaging, flow simulation, and protein adsorption experiments characterizes the foam’s performance.
Chinmaya Prerana Inguva, Joseph Lavoie, Afsaneh Rabiei   +2 more
wiley   +1 more source

Characterization of the galactomannans from Parkinsonia aculeata seeds and their application on affinity chromatography

Polímeros, 2006
Successive aqueous (10 ºC, 25 ºC and 80 ºC) and alkaline (1M NaOH; 25 ºC) extractions of milled Parkinsonia aculeata endosperms gave rise to four galactomannan fractions. These extractions furnished viscous galactomannans with Man:Gal ratios ranging from
Ivone Garros-Rosa, Fany Reicher, Carmen L. O. Petkowicz, Maria Rita Sierakowski, Renato A. Moreira   +4 more
doaj   +1 more source

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source

Programming Nanoscale Reaction Environments via Peptide Localization for Gold Nanoparticle Growth in Liposomal Nanoreactors

Advanced Functional Materials, EarlyView.
The liposome nanoreactor method enables uniform metal nanoparticle synthesis using the interior as a reaction environment. We demonstrate that peptide sequences and lipid compositions act as a functional molecular switch to control reaction environments in liposome nanoreactors.
Yuya Abe, Joseph Fox, Zabeada Aslam, Kevin Critchley, Stephen D. Evans, Mina Okochi, Masayoshi Tanaka   +6 more
wiley   +1 more source

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

Frontiers in Chemistry, 2019
Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational ...
Marcela Cristina de Moraes, Carmen Lucia Cardoso, Quezia Bezerra Cass   +2 more
doaj   +1 more source

Biomimetic Core–Shell Liposome Nanoparticles Deplete Lipoproteins and Enhance the Purity of Extracellular Vesicles from Human Blood

Advanced Functional Materials, EarlyView.
Lipoprotein‐mimicking nanoparticles (SL‐NPs), comprising Fe3O4@SiO2 cores coated with liposomal bilayers, selectively capture lipoproteins through protein corona formation. The resulting SL‐NP‐lipoprotein complexes can be removed by low‐speed centrifugation, thereby enhancing the purity of small extracellular vesicles (sEVs) isolated from human serum ...
Man‐Di Wang, Andrew J. Perciaccante, David Inman, Bong Hwan Sung, Alissa M. Weaver, Suzanne M. Ponik, Ying Ge, Song Jin   +7 more
wiley   +1 more source

Myogenic Fusogen‐Engineered Lipid Nanoparticles Enhance mRNA Delivery in Skeletal Muscle

Advanced Functional Materials, EarlyView.
This study reports a biomimetic strategy of engineering full‐length Myomaker, a muscle‐specific fusogen, into lipid nanoparticles (LNPs) to harness the native myoblast fusion capability for skeletal muscle mRNA delivery. The resulting Mymk‐LNPs enhance transfection in differentiating myocytes and enable Cre‐mediated reporter activation in injured ...
Fangyu Zhang, Zhisheng Xu, Yaou Duan, Yehui Sun, Shiying Wu, Tao Long, Mateusz Durbacz, Li Zhang, Yufen Xiao, Yu‐Chung Pien, Erick D. Guerrero, Zeru Tian, Xu Wang, Pratima Basak, Sumanta Chatterjee, Ning Liu, Xiaochun Li, Eric N. Olson, Daniel J. Siegwart   +18 more
wiley   +1 more source

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

MethodsX
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza, Roberto Verardo, Claudio Schneider   +2 more
doaj   +1 more source

Photo‐Switchable Molecular Module Programming PET Biodegradation in Aquatic Environments

Advanced Functional Materials, EarlyView.
We address the plastic durability paradox by integrating an itaconic acid–derived pyrrolidone module into polymer backbones. Sunlight in water triggers Norrish Type I ring‐opening and hydrophilization, enabling rapid biodegradation into nontoxic minerals.
Mohammad Asif Ali, Jixin Zheng, Satoshi Wakai, Jie Liu, Hidetaka Nomaki, Misato Tsukatani, Noriyuki Isobe, Jingcheng Sang, Yuriko Aoki, Hideo Okamura, Daisuke Sasaki, Chiaki Ogino, Yuzo Yamazaki, Takashi Nishikaze, Kenji Takada, Maiko K. Okajima, Tatsuo Kaneko   +16 more
wiley   +1 more source

Protein A immobilized monolithic capillary column for affinity chromatography

, 2002
Monolithic capillary columns for affinity chromatography were prepared by an in situ polymerization procedure using glycidyl methacrylate (GMA) as a monomer and trimethylolpropane trimethacrylate (TRIM) and ethylene dimethacrylate (EDMA) as cross-linkers,
Zou, HF, Wu, RN, Pan, ZF, Huang, XD, Mo, WM   +4 more
core