AChE was purified by affinity chromatography from Locusta migratoria head. The purification factors and yields were 474.81fold and 28.93%, respectively. The molecular weight of the purified enzyme was 67.22kDa, measured by SDSPAGE.
DENG Yuan-Jie +5 more
doaj
Selection of bispecific antibodies with optimal developability using FcRn‑Ph‑HPLC as an optimized FcRn affinity chromatography method. [PDF]
Müller T +6 more
europepmc +1 more source
Concanavalin A-Nonbinding Enzymes of Crotalus scutulatus scutulatus Venom [PDF]
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The Concanavalin A-nonbinding fraction (F-l) exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC ...
Childs, C. K. +3 more
core
PET Imaging of Cardiac Inflammation in Viral Myocarditis Using a DPP4‐Targeted Probe
This study describes a DPP4‐targeted PET probe for imaging myocardial inflammation by selectively targeting activated immune cells. Derived from the clinically approved small‐molecule inhibitor linagliptin, the probe demonstrates favorable biodistribution with specific cardiac uptake in myocarditis.
Wanhao Gao +14 more
wiley +1 more source
Analysis of Proteins and Peptides of Highly Purified CD9+ and CD63+ Horse Milk Exosomes Isolated by Affinity Chromatography. [PDF]
Sedykh SE +5 more
europepmc +1 more source
Canagliflozin treatment reshapes the gut microbiota in DKD and elevates levels of melibiose, a metabolite derived from Roseburia intestinalis. Melibiose directly binds to and enhances the enzymatic activity of glyoxalase 1, leading to decreased methylglyoxal accumulation.
Wei Zhang +32 more
wiley +1 more source
Use of tandem-affinity chromatography to incorporate nucleotide loading of small GTPases during their purification. [PDF]
Oladimeji R +4 more
europepmc +1 more source
ABSTRACT Gut microbiota dysbiosis promotes colorectal cancer (CRC) tumorigenesis. A global fecal metagenomic analysis identified Gemella morbillorum as a key contributor to the CRC‐associated microbiota. Fluorescence in situ hybridization revealed that Gemella morbillorum is enriched in CRC tumor tissues compared to adjacent normal tissues.
Zhen Wang +8 more
wiley +1 more source
Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications. [PDF]
Hage DS +8 more
europepmc +1 more source
Affinity Chromatography-Based Assays for the Screening of Potential Ligands Selective for G-Quadruplex Structures. [PDF]
Platella C +4 more
europepmc +1 more source

