Results 161 to 170 of about 533,930 (303)

DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling‐Mediated Anti‐tumor Immunity

Advanced Science, EarlyView.
Lung cancer remains the leading cause of cancer‐related death. We investigated the role of the epigenetic regulator DMAP1 in NSCLC and found that its loss induces replication stress and DNA damage. This in turn activates type I IFN signaling via the cGAS–STING pathway and transcriptional ISG de‐repression, enhancing anti‐tumor immune responses ...
Kan Huang, Xi Dai, Shuaihu Li, Yingxue Chen, Yaxin Yu, Lin Wang, Kun Liu, Shuhan Lyu, Chongyang Li, Yihua Sun, Fei Li   +10 more
wiley   +1 more source

Integrative structural insights into the IgG-FcRn interactions revealed by engineered FcRn-immobilized affinity chromatography. [PDF]

Commun Biol
Kiyoshi M, Suzuki T, Inoue N, Otake R, Yumoto T, Hirono L, Terao Y, Shibata H, Ide T, Nagatoishi S, Tsumoto K, Ishii-Watabe A.   +11 more
europepmc   +1 more source

Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography. [PDF]

Microb Cell Fact, 2023
Carratalá JV, Atienza-Garriga J, López-Laguna H, Vázquez E, Villaverde A, Sánchez JM, Ferrer-Miralles N.   +6 more
europepmc   +1 more source

The AUTACE That Degrades KRAS and Engages CD8+ T Cells for the Treatment of KRAS/TP53 Co‐Mutant Tumors

Advanced Science, EarlyView.
AUTACE is a bifunctional nanoplatform that integrates tumor targeting, immune engagement, and on‐demand KRAS degradation. It targets KRAS/TP53 co‐mutant tumors via TP53‐specific TCRs, elicits antitumor CD8+ T‐cell responses through surface anti‐CD3 antibodies, and uses low‐intensity focused ultrasound (LIFU) to trigger controlled release of the KRAS ...
Luo Li, Bolin Li, Yanan Hao, Bo Pang, Pan Li, Chunli Li, Yu Tang   +6 more
wiley   +1 more source

Selection of Functional Glycoforms in Anti-SARS-CoV-2 Human IgG1 Monoclonal Antibodies by FcγRIIIa Affinity Chromatography and Mass Spectrometry. [PDF]

J Med Chem
Oliviero B, Kumar S, Conteianni D, Donetti G, Cerino A, Iraci AS, La Gaipa A, Ottolini S, Tengattini S, Massolini G, Cassaniti I, Sammartino JC, Mele D, Baldanti F, Forneris F, Faravelli S, Scotti C, Pessino G, Maggi M, Mantovani S, Temporini C, Mondelli MU, Terreni M.   +22 more
europepmc   +1 more source

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification. [PDF]

Int J Pept Res Ther, 2021
Barredo-Vacchelli GR, Giudicessi SL, Martínez-Ceron MC, Cascone O, Camperi SA.   +4 more
europepmc   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

Protocol for recombinant expression in E. coli and purification of iBoost vaccine proteins using immobilized metal affinity chromatography. [PDF]

STAR Protoc
Huijbers EJM, van Beijnum JR, Koetsier J, Wachholz GE, Kemper PCJ, Myburgh L, Meister S, van Loon K, Cassee MA, Griffioen AW.   +9 more
europepmc   +1 more source

Pilot investigation of magnetic nanoparticle-based immobilized metal affinity chromatography for efficient enrichment of phosphoproteoforms for mass spectrometry-based top-down proteomics. [PDF]

Anal Bioanal Chem, 2023
Wang Q, Fang F, Sun L.
europepmc   +1 more source

Subunit interactions within the Saccharomyces cerevisiae DNA polymerase ε (pol ε) complex - Demonstration of a dimeric pol ε [PDF]

Saccharomyces cerevisiae DNA polymerase epsilon (pol ε) is essential for chromosomal replication. A major form of pol ε purified from yeast consists of at least four subunits: Pol2p, Dpb2p, Dpb3p, and Dpb4p.
Campbell, Judith L., Dua, Rajiv, Edwards, Shaune, Levy, Daniel L.   +3 more
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