Results 181 to 190 of about 533,930 (303)

Enriching Cysteine-Containing Peptides Using a Sulfhydryl-Reactive Alkylating Reagent with a Phosphonic Acid Group and Immobilized Metal Affinity Chromatography. [PDF]

J Proteome Res, 2023
Liu X, Rossio V, Gygi SP, Paulo JA.
europepmc   +1 more source

Quantitative Mass Spectrometry‐Based Biodistribution of Monoclonal Antibodies: An Alternative to Radio‐Biodistribution

Advanced Science, EarlyView.
Monoclonal antibodies are powerful anticancer therapeutics targeting tumor‐associated antigens. Mass spectrometry enables precise and accurate quantification of mAbs in tumor plasma and healthy organs. Unlike ligand binding assay and radioactivity, mass spectrometry does not suffer from matrix effects and labelling artefacts while facilitating method ...
Domenico Ravazza, Sheila Dakhel Plaza, Samuele Cazzamalli, Andrea Ciamarone, Andrea Galbiati, Riccardo Stucchi, Frederik Peissert, Emanuele Puca, Dario Neri, Ettore Gilardoni   +9 more
wiley   +1 more source

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification. [PDF]

Biotechnol Bioeng
Wu Y, Barbieri E, Smith WK, Minzoni A, Kilgore RE, Chu W, Daniele MA, Menegatti S.   +7 more
europepmc   +1 more source

[Preparation of a block copolymer-based temperature-responsive affinity chromatography stationary phase for antibody separation and purification]. [PDF]

Se Pu, 2023
Guo D, Xia Y, Mujeeb UR, Wang J, Liu J, Bai Q.   +5 more
europepmc   +1 more source

Disruption of Treg Homeostasis in Rheumatoid Arthritis via Ferroptosis‐Mediated ETC Collapse and TXK‐STAT3/PLCγ1 Activation

Advanced Science, EarlyView.
In rheumatoid arthritis, synovial Tregs accumulate but are functionally impaired due to iron overload‐induced ferroptosis. This triggers mitochondrial dysfunction and TXK tyrosine kinase‐mediated signaling, leading to Treg destabilization and inflammation.
Jingrong Chen, Xiao Guan, Kexu Xiong, Xiaoyi Shi, Luyao Wu, Chuanxin Su, Jun Zhao, Rongzhen Liang, Huimin Wang, Junlong Dang, Ye Chen, Yiding Xiong, Chi Zhou, Futao Zhao, Zhenhang Zhu, Xiaoli Fan, Li Zhou, Nancy Olsen, Yutong Jiang, Song Guo Zheng   +19 more
wiley   +1 more source

Studies and analysis of drug-target interactions by affinity chromatography and related techniques: A review. [PDF]

J Pharm Anal
Hage DS, Sharmeen S, Sajeeb BK, Olupathage H, Rahman MM, Kyei I, Alim S, Pinky NS.   +7 more
europepmc   +1 more source

Combining IP₃ affinity chromatography and bioinformatics reveals a novel protein-IP₃ binding site on Plasmodium falciparum MDR1 transporter. [PDF]

Curr Res Microb Sci, 2023
Alves E, Nakaya H, Guimarães E, Garcia CRS.   +3 more
europepmc   +1 more source

Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis

Advanced Science, EarlyView.
In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley   +1 more source

Limitations of boronate affinity chromatography for the specific enrichment of fructose-derived early glycation products in protein analytics. [PDF]

Anal Bioanal Chem
Lux S, Vogt C, Voll M, Hoffmann R.
europepmc   +1 more source

Tailored Porous Bimetallic Nanozyme Platform for Full‐Cycle Therapeutics of Intestinal Ischemia/Reperfusion

Advanced Science, EarlyView.
ABSTRACT Intestinal ischemia/reperfusion (I/R) injury presents a biphasic pathology: an acute oxidative‐inflammatory phase leading to organ failure, and a recovery phase marked by mucosal dysfunction and bacterial translocation. The developed MPB@TA‐Cu‐Ma nanocomposite functions as a dual‐phase therapeutic platform with significant efficacy. It rapidly
Chenghao Qiu, Wenjing Xian, Mengxue Wang, Guozhi Zhao, Yanhuo Zhang, Zhiyu Zhou, Bo Sheng, Zhuo Zhen, Xie Liu, Shouwen Diao, Mengwei Tu, Chen Qiu, Huixian Wang, Jianbo Zhang, Peng Zhu, Tong Li   +15 more
wiley   +1 more source