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Electron microscopic study of the formation of African horse-sickness virus

Archiv f�r die gesamte Virusforschung, 1967
BHK 21/13 cells infected with African horse-sickness virus (strain 13/63, type 3) for varying periods of time have been studied with the electron microscope. Evidence is presented to show that the virus does not multiply in the nucleus, but that replication occurs in the cytoplasmic matrix.
G, Lecatsas, B J, Erasmus
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Epizootiology and vectors of African horse sickness virus

Comparative Immunology, Microbiology and Infectious Diseases, 1994
African horse sickness (AHS) virus causes a non-contagious, infectious, arthropod-borne disease of equines and is enzootic in sub-Saharan Africa. The major vectors are species of Culicoides but mosquitoes and ticks may be involved. Periodically the virus makes excursions beyond its enzootic zones but until recently has not been able to maintain itself ...
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Detection of African horse sickness virus by reverse transcription-PCR

Journal of Clinical Microbiology, 1994
Reverse transcription-PCR (RT-PCR) was used to detect African horse sickness virus (AHSV). A single primer pair which amplified a 423-bp fragment of the S8 gene which encodes the NS2 protein of AHSV was identified. Amplification of this fragment from all nine serotypes of AHSV was achieved with these primers.
M, Stone-Marschat   +3 more
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Bluetongue, Schmallenberg virus… African horse sickness?

Veterinary Record, 2014
FOLLOWING the incursions into the UK of bluetongue virus (BTV) in 2007 and Schmallenberg virus (SBV) in 2011, an increasingly pertinent question for those tasked with predicting and responding to outbreaks of livestock arboviruses in the UK is, what is next?
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Factors associated with improved haemagglutination by African horse sickness virus

Veterinary Microbiology, 1982
Abstract Concentrated cell culture fluids of African horse sickness virus were shown to agglutinate erythrocytes from cattle, horses, sheep, goats, guinea pigs, rabbits, and poultry at 4°C, room temperature, and 37°C. The titres obtained were dependent on pH and NaCl molarity of the diluent, optimal titres being obtained at pH 7.5 and 0.6 M NaCl. The
S, Tokuhisa, Y, Inaba, K, Sato
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Hæmagglutination and Hæmagglutination-Inhibition with African Horse-Sickness Virus

Nature, 1961
COMPLEMENT-fixation and mouse neutralization tests are the two techniques so far employed in serological work with African horse-sickness virus. It was thought worth while to study the haemagglutinating properties of this agent with the view of obtaining yet another tool for epidemiological (epizootiological) studies of this dreaded horse disease.
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An Improved Diluent for the Production of African Horse-sickness Virus Vaccine

Nature, 1951
IT is the general practice among virologists to use 10 per cent normal serum saline as the diluent of choice for the preparation of virus emulsions. In keeping with this practice, horse-sickness vaccine consists essentially of suitably prepared emulsions of selected virus strains in 10 per cent normal horse serum diluted in the same fluid to contain ...
A, POLSON   +3 more
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Inactivated African horse sickness virus cell culture vaccine.

Immunology, 1968
Immunogenic killed vaccine against African Horse Sickness can be prepared from a neurotropic vaccine strain or a virulent strain of virus, type 9, grown in a monkey kidney stable cell line. Virus was inactivated with either formaldehyde in a final concentration of 1:8000 or β-propiolactone at 0·2 per cent.
MIRCHAMSY, H., TASLIMI, H.
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Inactivation of African horse-sickness virus by betapropiolactone and by pH

Archives of Virology, 1975
The inactivation of several types of African horse sickness virus (AHSV) by pH and by betapropiolactone (BPL) was studied. At 19 degrees - 22 degrees C the virus was stable between pH 6.0 and 10.4, whether suspended in mouse brain or in serumfree buffer.
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The Application of Fluorescent Antibody Techniques to the Virus ot African Horse Sickness

Research in Veterinary Science, 1974
Summary Direct anil indirect fluorescent antibody techniques teere successfully applied to the identification of virus isolates in tissue culture and for the screening of sera for antibody to the virus. The test was shown to be group specific. Antibody ivas shown by this method to be 1 widespread in wild zebra in Kenya.
F G, Davies, L J, Lund
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