Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Individual participant data meta-analysis versus aggregate data meta-analysis: A case study in eczema and food allergy prevention. [PDF]
Clin Exp Allergy, 2022 Van Vogt E +7 more
europepmc +1 more source
Simulation studies to compare bayesian wavelet shrinkage methods in aggregated functional data [PDF]
, 2022 Alex Rodrigo dos Santos Sousa
openalex +1 more source
Combining disaggregate forecasts or combining disaggregate information to forecast an aggregate [PDF]
To forecast an aggregate, we propose adding disaggregate variables, instead of combining forecasts of those disaggregates or forecasting by a univariate aggregate model. New analytical results show the effects of changing coefficients, mis-specification,
Hendry, David F., Hubrich, Kirstin
core
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Spatial Disaggregation of Agricultural Production Data [PDF]
In this paper we develop a dynamic data-consistent way for estimating agricultural land use choices at a disaggregate level (district-level), using more aggregate data (regional-level). The disaggregation procedure requires two steps.
Howitt, Richard E., Reynaud, Arnaud
core +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Pharmacometric estimation methods for aggregate data, including data simulated from other pharmacometric models. [PDF]
J Pharmacokinet Pharmacodyn, 2021 Välitalo PAJ.
europepmc +1 more source
Are Cyclical Fluctuations in Productivity Due More to Supply Shocks or Demand Shocks? [PDF]
Measured productivity is strongly procyclical. Real business cycle theories suggest that actual fluctuations in productivity are the source of fluctuations in aggregate output.
Matthew D. Shapiro
core
FEBS Letters, EarlyView.Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley +1 more source